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Clinical Chemistry 45: 62-67, 1999;
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(Clinical Chemistry. 1999;45:62-67.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Evaluation of Interferences in Rate and Fixed-Time Nephelometric Assays of Specific Serum Proteins

Xavier Bossuyta and Norbert Blanckaert

Department of Clinical Pathology, University Hospital Leuven, Kapucijnenvoer 33, B-3000 Leuven, Belgium.
a Author for correspondence. Fax 32 16 332896; e-mail xavier.bossuyt{at}uz.kuleuven.ac.be.

We performed interference studies for IgG, IgA, IgM, haptoglobin, and {alpha}1-antitrypsin assayed in serum, using either fixed-time nephelometry on the BN 100 from Behring or rate nephelometry on two analyzers from Beckman Instruments. For clear serum samples, results for IgG, IgA, IgM, and haptoglobin obtained with the three nephelometers showed good agreement. Values for {alpha}1-antitrypsin in clear sera were lower with the BN 100 than with the Array 360 or Immage. In lipemic samples, the BN 100 gave higher values than the Array 360 or Immage for all analytes except IgG. Addition of Intralipid to serum produced atypical reactions with the BN 100 (fixed-time nephelometry) but not with the Array 360 or Immage (rate nephelometry). The interference of lipemia on the BN 100 was also seen when the Beckman antibody was used, indicating that the effect was reagent-independent. For hemolyzed samples, the BN 100 gave higher values than the Array 360 or Immage for haptoglobin but not for the other analytes. Addition of increasing amounts of a hemolysate to serum revealed a negative interference in all assay systems. This effect was more pronounced with the Beckman reagent than with the Behring reagent in all three nephelometers and was independent of the type of instrument (fixed-time vs rate nephelometry).




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
E. B. Duly, G. Barnes, S. Grimason, and T. R. Trinick
Analytical Performance of Specific-Protein Assays on the Abbott Aeroset System
Clin. Chem., September 1, 2001; 47(9): 1709 - 1710.
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