Clinical Chemistry
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Clinical Chemistry 45: 68-77, 1999;
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(Clinical Chemistry. 1999;45:68-77.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Evaluation of Nicked Human Chorionic Gonadotropin Content in Clinical Specimens by a Specific Immunometric Assay

Galina Kovalevskaya1,a, Steven Birken2, Tatsu Kakuma4, John Schlatterer1 and John F. O'Connor1,3

1 Irving Center for Clinical Research, and Departments of
2 Medicine and
3 Pathology, Columbia University College of Physicians and Surgeons, New York, NY 10032.

4 New York Hospital-Cornell Medical Center, White Plains, NY 10605.
a Address correspondence to this author at: Irving Center for Clinical Research, Columbia University College of Physicians and Surgeons, 630 West 168th St., PH10-305, New York, NY 10032. Fax 212-305-3213; e-mail gk49{at}columbia.edu.

We report the development and characterization of an IRMA for the direct measurement of nicked human chorionic gonadotropin (hCGn) in blood and urine. hCGn derived from a reference preparation of hCG used as an immunogen elicits monoclonal antibodies (mAbs) with enhanced recognition of human luteinizing hormone epitopes. The most specific assay for pregnancy hCGn is an IRMA composed of one mAb to choriocarcinoma-derived hCGn (C5) and a second mAb developed from immunization with normal-pregnancy hCGn. This assay was used to evaluate hCGn profiles in normal, in vitro fertilization, Down syndrome, and ectopic pregnancies. In all pregnancies, hCGn was usually present in much lower concentrations than the non-nicked hCG isoform. Our results suggest that some form of physical separation from the overwhelming quantities of non-nicked hCG present in clinical specimens will be required before accurate immunochemical estimations of hCGn can be made.




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R. McChesney, A. J. Wilcox, J. F. O'Connor, C. R. Weinberg, D. D. Baird, J. P. Schlatterer, D.R. McConnaughey, S. Birken, and R. E. Canfield
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M. Maccarrone, T. Bisogno, H. Valensise, N. Lazzarin, F. Fezza, C. Manna, V. Di Marzo, and A. Finazzi-Agro
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M. Maccarrone, H. Valensise, M. Bari, N. Lazzarin, C. Romanini, and A. Finazzi-Agro
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P. Mock, G. Kovalevskaya, J. F. O'Connor, and A. Campana
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