Clinical Chemistry
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Clinical Chemistry 45: 78-84, 1999;
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(Clinical Chemistry. 1999;45:78-84.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Immunoreactivity, Ultrastructural Localization, and Transcript Expression of Prostate-specific Antigen in Human Neuroblastoma Cell Lines

Ferdinando Mannello1,a, Manuela Malatesta1, Francesca Luchetti2, Stefano Papa2, Serafina Battistelli1 and Giancarlo Gazzanelli1

1 Institute of Histology and Laboratory Analysis, Faculty of Sciences, and
2 Institute of Morphological Sciences, University of Urbino, 61029 Urbino (PS), Italy.
a Address correspondence to this author at: Istituto di Istologia ed Analisi di Laboratorio, Facoltá di Scienze Matematiche, Fisiche e Naturali, Via E. Zeppi, 61029 Urbino (PS), Italy. Fax 39-722-322370; e-mail mannello{at}bio.uniurb.it.

Prostate-specific antigen (PSA) is considered a highly specific biochemical marker of the human prostate gland, and it currently is used for prostate cancer diagnosis and monitoring. Recently, PSA production and secretion were found in nondiseased and diseased cells, tissues, and fluids from women. In this study, we characterized the presence of PSA in two human neuroblastoma cell lines with biochemical, ultrastructural, and molecular approaches. Using reverse transcription-PCR, we identified PSA mRNA, and Western blotting revealed a substantial amount of complexed form of PSA protein, which is localized mainly in free ribosomes. Although the role of PSA in human neuroblastoma cell lines is still unknown, our study supports the hypothesis that this serine protease may be involved in controlling the growth of human brain tumor cells, adding more support to the notion that PSA is a widespread kallikrein-like protease with biological functions much more complex than recently thought.




The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
F. Mannello and M. Sebastiani
Zymographic Analyses and Measurement of Matrix Metalloproteinase-2 and -9 in Nipple Aspirate Fluids
Clin. Chem., September 1, 2003; 49(9): 1546 - 1550.
[Full Text] [PDF]

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 J. Clin. Endocrinol. Metab.Home page
M. Malatesta, F. Mannello, F. Luchetti, F. Marcheggiani, L. Condemi, S. Papa, and G. Gazzanelli
Prostate-Specific Antigen Synthesis and Secretion by Human Placenta: A Physiological Kallikrein Source during Pregnancy
J. Clin. Endocrinol. Metab., January 1, 2000; 85(1): 317 - 321.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
F. Mannello, M. Malatesta, M. Sebastiani, S. Battistelli, and G. Gazzanelli
Molecular Forms and Ultrastructural Localization of Prostate-specific Antigen in Nipple Aspirate Fluids,
Clin. Chem., December 1, 1999; 45(12): 2263 - 2266.
[Full Text] [PDF]

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 Clin. Chem.Home page
F. Mannello, S. Barulli, M. Malatesta, S. Mancini, P. Leoni, and G. Gazzanelli
Prostate-specific Antigen Expression in Normal Human Bone Marrow Cells
Clin. Chem., July 1, 1999; 45(7): 1102 - 1103.
[Full Text] [PDF]


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