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Whole Blood Capcellia CD4/CD8 Immunoassay for Enumeration of CD4+ and CD8+ Peripheral T Lymphocytes

¹ Ligne de Recherche en Immunologie, Sanofi Recherche, 371 rue du professeur Joseph Blayac, 34184 Montpellier Cedex 04, France.

² Laboratoire d'Immunologie des Infections Rétrovirales, Hôpital Lapeyronie, 34295 Montpellier, France.

³ Département des Maladies Infectieuses et Tropicales, Hôpital Gui de Chauliac, 34295 Montpellier, France.

⁴ Sanofi Diagnostics Pasteur, 92430 Marnes la Coquette, France.

⁵ Unité Mixte de Recherche 9921, Faculté de Pharmacie, 34060 Montpellier, France.
^a Author for correspondence. Fax 33 4 67 10 60 00; e-mail dominique. carriere{at}sanofi.com.

We evaluated the Whole Blood Capcellia^® CD4/CD8, an immunoenzymatic method that provides absolute counts of CD4+ and CD8+ T cells in peripheral blood. The assay is based on the separation of T cells by use of an anti-CD2 magnetic bead suspension, followed by reaction of the CD4 or CD8 molecules with the corresponding monoclonal antibody coupled to peroxidase. CD4-positive monocytes were excluded from the assay. Freeze-dried magnetic bead-T-cell complexes were used as calibrators. Capcellia counts from HIV-1-infected patients were compared with those obtained by flow cytometry as the comparison method. The results by Capcellia correlated well with those by flow cytometric analysis: r² = 0.95; P <0.001; (y = 0.96x - 22.1); S_y|x = 64 for CD4; r² = 0.81; P <0.001; (y = 1.26x - 76.4); S_y|x = 139 for CD8; n = 76. The correlation between CD4+ T-cell counts determined by two trained experimenters was significant (r² = 0.96). Our results indicate that this new ELISA technique for lymphocyte immunophenotyping is an efficient alternative to flow cytometry.

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