Clinical Chemistry
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Clinical Chemistry 45: 92-97, 1999;
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(Clinical Chemistry. 1999;45:92-97.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Whole Blood Capcellia CD4/CD8 Immunoassay for Enumeration of CD4+ and CD8+ Peripheral T Lymphocytes

Dominique Carrière1,a, Jean Pierre Vendrell2, Claude Fontaine1, Aline Jansen1, Jacques Reynes3, Isabelle Pagès2, Catherine Holzmann4, Michel Laprade1 and Bernard Pau5

1 Ligne de Recherche en Immunologie, Sanofi Recherche, 371 rue du professeur Joseph Blayac, 34184 Montpellier Cedex 04, France.

2 Laboratoire d'Immunologie des Infections Rétrovirales, Hôpital Lapeyronie, 34295 Montpellier, France.

3 Département des Maladies Infectieuses et Tropicales, Hôpital Gui de Chauliac, 34295 Montpellier, France.

4 Sanofi Diagnostics Pasteur, 92430 Marnes la Coquette, France.

5 Unité Mixte de Recherche 9921, Faculté de Pharmacie, 34060 Montpellier, France.
a Author for correspondence. Fax 33 4 67 10 60 00; e-mail dominique. carriere{at}sanofi.com.

We evaluated the Whole Blood Capcellia® CD4/CD8, an immunoenzymatic method that provides absolute counts of CD4+ and CD8+ T cells in peripheral blood. The assay is based on the separation of T cells by use of an anti-CD2 magnetic bead suspension, followed by reaction of the CD4 or CD8 molecules with the corresponding monoclonal antibody coupled to peroxidase. CD4-positive monocytes were excluded from the assay. Freeze-dried magnetic bead-T-cell complexes were used as calibrators. Capcellia counts from HIV-1-infected patients were compared with those obtained by flow cytometry as the comparison method. The results by Capcellia correlated well with those by flow cytometric analysis: r2 = 0.95; P <0.001; (y = 0.96x - 22.1); Sy|x = 64 for CD4; r2 = 0.81; P <0.001; (y = 1.26x - 76.4); Sy|x = 139 for CD8; n = 76. The correlation between CD4+ T-cell counts determined by two trained experimenters was significant (r2 = 0.96). Our results indicate that this new ELISA technique for lymphocyte immunophenotyping is an efficient alternative to flow cytometry.




The following articles in journals at HighWire Press have cited this article:


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P. Nouanthong, S. Pata, T. Sirisanthana, and W. Kasinrerk
A Simple Manual Rosetting Method for Absolute CD4+ Lymphocyte Counting in Resource-Limited Countries.
Clin. Vaccine Immunol., May 1, 2006; 13(5): 598 - 601.
[Abstract] [Full Text] [PDF]

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R. Kannangai, S. Ramalingam, M. V. Jesudason, T. S. Vijayakumar, O. C. Abraham, A. Zachariah, and G. Sridharan
Correlation of CD4+ T-Cell Counts Estimated by an Immunocapture Technique (Capcellia) with Viral Loads in Human Immunodeficiency Virus-Seropositive Individuals
Clin. Vaccine Immunol., November 1, 2001; 8(6): 1286 - 1288.
[Abstract] [Full Text] [PDF]


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