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Clinical Chemistry 45: 1938-1943, 1999;
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(Clinical Chemistry. 1999;45:1938-1943.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Plastic Microchip Electrophoresis for Analysis of PCR Products of Hepatitis C Virus

Yu-Hung Chen1, Wei-Chang Wang1, Kung-Chia Young2, Ting-Tsung Chang3 and Shu-Hui Chen1,a

1 Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan.
Departments of
2 Medical Technology and
3 Internal Medicine, National Cheng Kung University Hospital, Tainan 701, Taiwan.
a Author for correspondence. Fax 886-6-2740552; e-mail shchen{at}mail.ncku.edu.tw

Background: Electrophoresis on polymeric rather than glass microstructures is a promising separation method for analytical chemistry. Assays on such devices need to be explored to allow assessment of their utility for the clinical laboratory.

Methods: We compared capillary and plastic microchip electrophoresis for clinical post-PCR analysis of hepatitis C virus (HCV). For capillary electrophoresis (CE), we used a separation medium composed of 10 g/L hydroxypropyl methyl cellulose in Tris-borate-EDTA buffer and 10 µmol/L intercalating dye. For microchip electrophoresis, the HCV assay established on the fused silica tubing was transferred to the untreated polymethylmethacrylate microchip with minimum modifications.

Results: CE resolved the 145-bp amplicon of HCV in 15 min. The confidence interval of the migration time was <3.2%. The same HCV amplicon was resolved by microchip electrophoresis in <1.5 min with the confidence interval of the migration time <1.3%.

Conclusion: The polymer microchip, with advantages that include fast processing time, simple operation, and disposable use, holds great potential for clinical analysis.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
S. F.Y. Li and L. J. Kricka
Clinical Analysis by Microchip Capillary Electrophoresis
Clin. Chem., January 1, 2006; 52(1): 37 - 45.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Ferrance, K. Snow, and J. P. Landers
Evaluation of Microchip Electrophoresis as a Molecular Diagnostic Method for Duchenne Muscular Dystrophy
Clin. Chem., February 1, 2002; 48(2): 380 - 383.
[Full Text] [PDF]


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