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Clinical Chemistry 45: 2183-2190, 1999;
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(Clinical Chemistry. 1999;45:2183-2190.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Preanalytical Variables Affecting the Quantification of Fatty Acid Ethyl Esters in Plasma and Serum Samples

Britt L. Soderberg1, Ewa T. Sicinska1, Emily Blodget1, Joanne E. Cluette-Brown1, Paolo M. Suter2, Theresa Schuppisser2, Wilhem Vetter2 and Michael Laposata1,a

1 Division of Laboratory Medicine, Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114.

2 Medical Policlinic, University Hospital, Ramistrasse 100, CH-8091, Zurich, Switzerland.
a Address correspondence to this author at: Room 235, Gray Bldg., Massachusetts General Hospital, Boston, MA 02114. Fax 617-726-3256; e-mail mlaposata{at}partners.org

Background: Fatty acid ethyl esters (FAEEs) are cytotoxic nonoxidative ethanol metabolites produced by esterification of fatty acids and ethanol. FAEEs are detectable in blood up to 24 h after ethanol consumption. The objective of this study was to assess the impact of gender, serum or plasma triglyceride concentration, time and temperature of specimen storage, type of alcoholic beverage ingested, and the rate of ethanol consumption on FAEE concentrations in plasma or serum.

Methods: For some studies, subject were recruited volunteers; in others, residual blood samples after ethanol quantification were used. FAEEs were isolated by solid-phase extraction and quantified by gas chromatography–mass spectrometry.

Results: For weight-adjusted amounts of ethanol intake, FAEE concentrations were twofold greater for men than women (P <=0.05). Accounting for triglycerides improved the correlation between blood ethanol concentrations and FAEE concentrations for both men (from r = 0.640 to r = 0.874) and women (from r = 0.619 to r = 0.673). FAEE concentrations did not change when samples were stored at or below 4 °C, but doubled when stored at room temperature for >=24 h. The type of alcoholic beverage and rate of consumption did not affect FAEE concentrations.

Conclusion: These studies advance plasma and serum FAEE measurements closer to implementation as a clinical test for ethanol intake.




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