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Clinical Chemistry 45: 2216-2223, 1999;
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(Clinical Chemistry. 1999;45:2216-2223.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Freedom from Drug Interference in New Immunoassays for Urinary Catecholamines and Metanephrines

Julie Wassell1,a, Paul Reed2, John Kane2 and Cyril Weinkove2

1 Department of Biochemistry, Wythenshawe Hospital, Southmoor Rd., Manchester M23 9LT, UK.

2 Department of Biochemistry, Hope Hospital, Stott Lane, Manchester M6 8HD, UK.
a Author for correspondence. Fax 0161-291-2125; e-mail juliewassell{at}hotmail.com

Background: Determination of urinary free catecholamine and total (i.e., free plus conjugated) metanephrine excretion is considered the most clinically sensitive biochemical test for pheochromocytoma. In this study, we evaluated new immunoassay methods for the measurement of these analytes for potential drug-based interference.

Methods: Urine samples collected from patients on a variety of medications were grouped by specific drug type. The significance of any difference in the free catecholamine or total metanephrine concentrations in the different groups was assessed by one-way ANOVA. A group of patients receiving no medication was included as a control (no analytical interference). Additionally, analytical accuracy, detection limit, and precision were determined.

Results: No significant differences were found in the concentrations of free catecholamines or total metanephrines in urine from patients taking the medications investigated and the control group: P = 0.649 (fE), 0.221 (fNE), 0.149 (tM), and 0.170 (tNM). For free catecholamines, intraassay CVs were 4.6–18%; interassay CVs were 10–25%. For total metanephrines, intraassay CVs were 9.6–27%; interassay CVs were 5.8–22%. Detection limits were 0.009 and 0.027 µmol/L for fE and fNE and 0.119 and 0.346 µmol/L for tM and tNM, respectively.

Conclusions: None of the drugs examined in this study interfered in the measurement of free catecholamines or total metanephrines by these immunoassays. The technique is easier to use, requires less equipment, and is more accessible than HPLC. In combination, these assays are suitable as initial screening tests for pheochromocytoma.




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R. L. Taylor and R. J. Singh
Validation of Liquid Chromatography-Tandem Mass Spectrometry Method for Analysis of Urinary Conjugated Metanephrine and Normetanephrine for Screening of Pheochromocytoma
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