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Evaluation of a Capillary Electrophoresis Method for Routine Determination of Hemoglobins A₂ and F

¹ Department of Clinical Chemistry, Hôpital Erasme, Université Libre de Bruxelles, 808 route de Lennik, B1070 Brussels, Belgium.

² Analis SA, 14 rue Dewez, B5000 Namur, Belgium.
^a Author for correspondence. Fax 32 2 555 6655; e-mail fcotton{at}ulb.ac.be.

Hemoglobin A₂ (Hb A₂) and hemoglobin F (Hb F) are important analytes in the diagnosis and follow up of Hb diseases. We evaluated a new capillary zone electrophoresis (CZE) kit for Hb A₂ and Hb F measurements. The imprecision ranged from 3% to 6% for Hb A₂ and Hb F at physiological and pathological concentrations. The method compared well with cation-exchange HPLC for Hb A₂ and Hb F and with anion-exchange chromatography in microcolumns (MAEC), for Hb A₂. Nevertheless, higher results were obtained [Hb A_{2 CZE} (%) = 1.233 Hb A_{2 HPLC} - 0.2; Hb A_{2 CZE} (%) = 1.190 Hb A_{2 MAEC} + 0.1; Hb F_CZE (%) = 1.118 Hb F_HPLC + 0.4], and new reference values had to be determined (Hb A₂ 2.7–3.8%; Hb F <1.2%). Quantification of Hb A₂ was not influenced by Hb S. Measurement of Hb F was accurate and precise except at low concentrations in Hb AS patients. This new CZE kit is rapid, precise, and reliable, and seems appropriate for use in clinical laboratories.

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