Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 45: 244-251, 1999;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (61)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohta, H.
Right arrow Articles by Kangawa, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohta, H.
Right arrow Articles by Kangawa, K.
Related Collections
Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1999;45:244-251.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

One-Step Direct Assay for Mature-type Adrenomedullin with Monoclonal Antibodies

Hideki Ohta1,a, Tetsuo Tsuji1, Shigeru Asai1, Kazuyuki Sasakura1, Hiroshi Teraoka1, Kazuo Kitamura2 and Kenji Kangawa3

1 Research and Development and Manufacturing Department for Diagnostics, Diagnostic Science Division, Shionogi & Co., Ltd., 2-5-1, Mishima, Settsu, Osaka 566-0022, Japan.

2 First Department of Internal Medicine, Miyazaki Medical College, 5200, Kihara, Kiyotake, Miyazaki 889-1601, Japan.

3 National Cardiovascular Center Research Institute, 5-7-1, Fujishirodai, Suita, Osaka 565-0873, Japan.
a Address correspondence to this author at: R & D Manufacturing Department for Diagnostics, Diagnostic Science Division, Shionogi & Co., Ltd., 2-5-1, Mishima, Settsu, Osaka 566-0022, Japan. Fax 81 6 6319 4109; e-mail hideki.ohta{at}shionogi.co.jp.

Adrenomedullin (AM) is a potent hypotensive peptide. Plasma contains mature-type AM (m-AM), which is amidated at the carboxy terminus, and an intermediate, AM-Gly. We developed a one-step two-site IRMA specific for determining human m-AM with monoclonal antibodies. The detection limit was 0.5 pmol/L, and the working range (CV <15%) was 1–300 pmol/L. Dilution of plasma samples showed good linearity. The recovery of added AM was 91–118%. The intra- and interassay imprecision values (CVs) were 4.4–8.2% and 5.5–8.3%, respectively. The assay had no cross-reactivity with AM-Gly or other peptides similar to AM. The mean (± SD) plasma human m-AM concentration of 61 healthy subjects was 1.18 ± 0.65 pmol/L. In conclusion, our IRMA makes it possible to specifically measure m-AM, using a small amount of plasma sample (0.2 mL) by a one-step overnight assay without prior extraction. Our simplified method would be suitable for clinical studies on AM, especially when large numbers of samples must be processed.




The following articles in journals at HighWire Press have cited this article:


Home page
 Cardiovasc ResHome page
T. Tsuruda, J. Kato, K. Hatakeyama, A. Yamashita, K. Nakamura, T. Imamura, K. Kitamura, T. Onitsuka, Y. Asada, and T. Eto
Adrenomedullin in mast cells of abdominal aortic aneurysm
Cardiovasc Res, April 1, 2006; 70(1): 158 - 164.
[Abstract] [Full Text] [PDF]

Home page
 HeartHome page
T Ishikawa, K Hatakeyama, T Imamura, K Ito, S Hara, H Date, Y Shibata, Y Hikichi, Y Asada, and T Eto
Increased adrenomedullin immunoreactivity and mRNA expression in coronary plaques obtained from patients with unstable angina
Heart, October 1, 2004; 90(10): 1206 - 1210.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
D. Yoshikawa, F. Kawahara, N. Okano, H. Hiraoka, Y. Kadoi, N. Fujita, T. Morita, and F. Goto
Increased Plasma Concentrations of the Mature Form of Adrenomedullin During Cardiac Surgery and Hepatosplanchnic Hypoperfusion
Anesth. Analg., September 1, 2003; 97(3): 663 - 670.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. Yamasaki, J. Kawai, T. Nakaoka, T. Ogita, A. Tojo, and T. Fujita
Adrenomedullin Overexpression to Inhibit Cuff-Induced Arterial Intimal Formation
Hypertension, February 1, 2003; 41(2): 302 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
K. Marutsuka, K. Hatakeyama, A. Yamashita, Y. Sato, A. Sumiyoshi, and Y. Asada
Adrenomedullin augments the release and production of tissue factor pathway inhibitor in human aortic endothelial cells
Cardiovasc Res, January 1, 2003; 57(1): 232 - 237.
[Abstract] [Full Text] [PDF]

Home page
 HeartHome page
K Tambara, M Fujita, N Nagaya, S Miyamoto, A Iwakura, K Doi, G Sakaguchi, K Nishimura, K Kangawa, and M Komeda
Increased pericardial fluid concentrations of the mature form of adrenomedullin in patients with cardiac remodelling
Heart, March 1, 2002; 87(3): 242 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
T. Tokura, H. Kinoshita, S. Fujimoto, S. Hisanaga, K. Kitamura, and T. Eto
Plasma levels of mature form of adrenomedullin in patients with haemodialysis
Nephrol. Dial. Transplant., April 1, 2001; 16(4): 783 - 786.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
S. Fujioka, T. Sasaki, K. Hirata, K. Okada, A. Takazawa, H. Sakahashi, and A. Toyama
The Relationship Between Plasma Concentration of Mature Adrenomedullin and Jugular Venous Oxygen Saturation During and After Cardiopulmonary Bypass
Anesth. Analg., April 1, 2001; 92(4): 837 - 841.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
Y. Tomoda, K. Kikumoto, Y. Isumi, T. Katafuchi, A. Tanaka, K. Kangawa, K. Dohi, and N. Minamino
Cardiac fibroblasts are major production and target cells of adrenomedullin in the heart in vitro
Cardiovasc Res, March 1, 2001; 49(4): 721 - 730.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association for Clinical Chemistry.