Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 45: 347-354, 1999;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Black, M. H.
Right arrow Articles by Diamandis, E. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Black, M. H.
Right arrow Articles by Diamandis, E. P.
Related Collections
Right arrow Proteomics and Protein Markers
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 1999;45:347-354.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Characterization of Monoclonal Antibodies for Prostate-specific Antigen and Development of Highly Sensitive Free Prostate-specific Antigen Assays

Margot H. Black1,2, C. Linda Grass1, Jari Leinonen3, Ulf-Hkan Stenman3 and Eleftherios P. Diamandis1,2,a

1 Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5.

2 Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada M5G 1X5.

3 Department of Clinical Chemistry, Helsinki University Central Hospital, Helsinki, Finland.
a Author for correspondence. Fax 416-586-8628; e-mail ediamandis{at}mtsinai.on.ca

Background: The recent elucidation of the importance of serological free prostate-specific antigen (PSA) in the diagnosis of prostate cancer has created a demand for immunoassays specific for free PSA.

Methods: We developed and characterized 11 monoclonal antibodies with high affinities for PSA (Kavalues from 1.1 x 108 to 1.8 x 1010L/mol), only 3 of which cross-react with human glandular kallikrein (hK2). Using these antibodies and PSA antibodies developed by others, in conjunction with time-resolved fluorometry, we developed ultrasensitive sandwich immunoassays specific for the free form of PSA.

Results: The analytical detection limit of these immunoassays is 0.001 µg/L. To our knowledge, this is the most sensitive free PSA assay reported to date. The free PSA immunoassays exhibit <1% cross-reactivity with PSA-{alpha}1-antichymotrypsin, show no cross-reactivity with hK2, and correlate well with established free PSA kits. The 11 antibodies developed by our group, in conjunction with 4 commercially available antibodies, were used to generate a putative epitope map of the PSA molecule.

Conclusion: The highly sensitive free PSA immunoassays may be used for measuring PSA subfractions in female serum, an application currently impossible with other reported free PSA immunoassays. © 1999 American Association for Clinical Chemistry




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
J. Leinonen, P. Wu, and U.-H. Stenman
Epitope Mapping of Antibodies against Prostate-specific Antigen with Use of Peptide Libraries
Clin. Chem., December 1, 2002; 48(12): 2208 - 2216.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
B. R. Grzeda, T. Le Bui, C. N. Warner, T. L. Pirucki, L. M. Dewey, M. Babich, and J. A. Maggiore
Measurement of Prostate-specific Antigen by Use of a Novel Blood Collection and Analytical System
Clin. Chem., August 1, 2002; 48(8): 1272 - 1278.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. Soukka, J. Paukkunen, H. Harma, S. Lonnberg, H. Lindroos, and T. Lovgren
Supersensitive Time-resolved Immunofluorometric Assay of Free Prostate-specific Antigen with Nanoparticle Label Technology
Clin. Chem., July 1, 2001; 47(7): 1269 - 1278.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
B. Rafferty, P. Rigsby, M. Rose, T. Stamey, and R. Gaines Das
Reference Reagents for Prostate-specific Antigen (PSA): Establishment of the First International Standards for Free PSA and PSA (90:10)
Clin. Chem., September 1, 2000; 46(9): 1310 - 1317.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. H. Black, M. Giai, R. Ponzone, P. Sismondi, H. Yu, and E. P. Diamandis
Serum Total and Free Prostate-specific Antigen for Breast Cancer Diagnosis in Women
Clin. Cancer Res., February 1, 2000; 6(2): 467 - 473.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association for Clinical Chemistry.