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Clinical Chemistry 45: 355-359, 1999;
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(Clinical Chemistry. 1999;45:355-359.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Variability of Glutathione S-Transferase {alpha} in Human Liver and Plasma

Theo P.J. Mulder, Daniel A. Court and Wilbert H.M. Petersa

Department of Gastroenterology, University Hospital St. Radboud, 6500 HB Nijmegen, The Netherlands.
a Address correspondence to this author at: Department of Gastroenterology, University Hospital St. Radboud, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. Fax 31-243540103; e-mail W.Peters{at}gastro.azn.nl

Background: Glutathione S-transferases are a family of enzymes involved in the binding, transport, and detoxification of a wide variety of endogenous and exogenous compounds. Little information is available about the variability of class {alpha} glutathione S-transferases in human liver, where they are highly expressed, or in serum.

Methods: Both total class {alpha} glutathione S-transferase (GST-{alpha}, composed of GSTA1-1, GSTA1-2, and GSTA2-2) as well as GSTA1-1 concentrations were measured by specific and sensitive ELISA in liver cytosols of 35 organ donors and in plasma samples of 350 healthy controls.

Results: The mean total GST-{alpha} and GSTA1-1 in liver cytosols were 25.1 ± 9.4 and 10.7 ± 5.3 µg/mg protein, respectively, and did not correlate with activities of aspartate aminotransferase or alanine aminotransferase. The mean total GST-{alpha} in liver was significantly higher in females compared with males (28.8 ± 10.0 vs 22.0 ± 7.8 µg/mg protein; P <0.05). In contrast, the median total GST-{alpha} in plasma was lower in females compared with males (2.0 and 2.8 µg/L, respectively; P <0.0001). The median ratios for GSTA1-1/total GST-{alpha} in liver and plasma were 0.42 and 0.58, respectively.

Conclusions: GSTA1-1 constitutes approximately one-half of the total amount of {alpha} class GSTs in human plasma and liver. Total GST-{alpha} values are higher in female liver but lower in plasma compared with the respective values in males. © 1999 American Association for Clinical Chemistry




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