Clinical Chemistry
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Clinical Chemistry 45: 400-405, 1999;
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(Clinical Chemistry. 1999;45:400-405.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

TE671 Cell-based ELISA for Anti-Acetylcholine Receptor Antibody Determination in Myasthenia Gravis

Diego Franciotta1,a, Gianvito Martino3, Elena Brambilla4, Elisabetta Zardini1, Vera Locatelli1, Alessandra Bergami3, Carmine Tinelli5, Gaetano Desina6 and Vittorio Cosi2

1 Laboratory of Neuroimmunology and
2 Division B, Istituto di Ricovero e Cura a Carattere Scientifico, Neurological Institute `Mondino', via Palestro 3, University of Pavia, 27100 Pavia, Italy.

3 Experimental Neuroimmunotherapy and
4 Neuroimmunology Unit, Department of Biotechnology, San Raffaele Scientific Institute, via Olgettina 58, 20132 Milan, Italy.

5 Unit of Biometric, Istituto di Ricovero e Cura a Carattere Scientifico, Policlinico S. Matteo, University of Pavia, 27100 Pavia, Italy.

6 `Casa Sollievo della Sofferenza', 71013 San Giovanni Rotondo (FG), Italy.
a Author for correspondence. Fax 39-0382-380286; e-mail francid{at}cpbim1.unipv.it

Background: Acetylcholine receptor (AChR) from human muscles is the antigen used currently in radioimmunoprecipitation assays (RIPAs) for the determination of anti-AChR antibodies in the diagnosis of myasthenia gravis (MG). Our aim was to develop and validate an ELISA using TE671 cells as the source of AChR.

Methods: After TE671 cell homogenization, the crude AChR extract was used for plate coating. Anti-AChR antibodies were determined in 207 MG patients and in 77 controls.

Results: The mean intra- and interassay CVs (for two samples with different anti-AChR antibody concentrations) were 9.7% and 15.7%, respectively. Test sensitivity and specificity, for generalized MG, were 79.5% (95% confidence interval, 72.8–85.0%) and 96.1% (89.0–99.1%). The detection limit was 2 nmol/L. Anti-AChR antibody concentrations from 53 MG patients, as tested with our ELISA, showed good agreement with an RIPA with a mean difference (SD) of 1.0 (5.6) nmol/L.

Conclusion: Our ELISA is a simple screening test for the diagnosis of MG and enables rapid and inexpensive patient follow-up. © 1999 American Association for Clinical Chemistry




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
J. Ricny, L. Simkova, and A. Vincent
Determination of Anti-Acetylcholine Receptor Antibodies in Myasthenic Patients by Use of Time-resolved Fluorescence
Clin. Chem., March 1, 2002; 48(3): 549 - 554.
[Abstract] [Full Text] [PDF]


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