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Clinical Chemistry 45: 520-525, 1999;
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(Clinical Chemistry. 1999;45:520-525.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Assessment of Serum Thyroxine Binding Capacity-dependent Biases in Free Thyroxine Assays

Nicos D. Christofides1,a, Ewan Wilkinson, Mary Stoddart, David C. Ray and Geoff J. Beckett

1 Research and Development, Ortho-Clinical Diagnostics, Cardiff Laboratories, Whitchurch, Forest Farm Estate, Cardiff CF4 7YT, Wales, UK.
a Author for correspondence. Fax 44 (0) 1222 526635; e-mail NCHRISTOFIDES{at}compuserve.com

Background: Free thyroxine (FT4) assays may exhibit biases that are related to serum T4 binding capacity (sBC). We describe two tests that can be used to assess the presence and magnitude of sBC-dependent biases in FT4 assays.

Methods: We used a direct equilibrium dialysis FT4 assay as the reference method and compared the results obtained with those of the FT4 assays under investigation, in patient sera having a wide range of sBC. We then compared the expected and observed FT4 results for sera diluted with an inert buffer. Because serum dilution causes a predictable decrease in sBC, an increasingly negative bias on progressive dilution is indicative of a sBC-dependent bias.

Results: The automated FT4 assay investigated (Vitros FT4) showed no demonstrable sBC-dependent bias by either test.

Conclusion: These two tests can be used to screen for sBC-dependent biases in FT4 assays.© 1999 American Association for Clinical Chemistry




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Free Thyroxine Measured by Equilibrium Dialysis and Nine Immunoassays in Sera with Various Serum Thyroxine-binding Capacities
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