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Clinical Chemistry 45: 561-566, 1999;
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(Clinical Chemistry. 1999;45:561-566.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Within- and Between-Subject Variation in Commonly Measured Anthropometric and Biochemical Variables

Adji Widjaja1, Richard J. Morris2, Jonathan C. Levy2,a, Keith N. Frayn3, Susan E. Manley2 and Robert C. Turner2

1 Department of Clinical Endocrinology, Hannover Medical School, 30625 Hannover, Germany.

2 Diabetes Research Laboratories and
3 Oxford Lipid Metabolism Groupwdef, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, UK.
a Author for correspondence. Fax 0044-1865-723884; e-mail jonathan.levy{at}drl.ox.ac.uk

Background: The biological variation of some commonly assessed metabolic variables in healthy subjects has not been studied extensively. The aim of the study was to assess, in 12 healthy subjects (6 male and 6 female; mean (SD) age; 22.7 (1.5) years) following an overnight fast, the day-to-day variation of body fat (impedance method), triglycerides, nonesterified fatty acid (NEFAs), glycerol, 3-hydroxybutyrate (3-OHB), lactate, glucose, insulin (RIA), C-peptide, and glucagon on 12 consecutive days.

Methods: Between- and within-subject coefficients of variation (CVG and CVW) were estimated using a random effects analysis of variance, and assay variation was subtracted to give the coefficient of within-subject biological variation (CVI). Individuality indices were calculated as CVW/CVG.

Results: The overall means, CVI, and individuality indices were as follows: for body fat, 24.2%, 10%, and 0.3; for triglycerides, 0.61 mmol/L, 21%, and 1.1; for NEFAs, 376 µmol/L, 45%, and 1.4; for glycerol, 48 µmol/L, 36%, and 0.8; for 3-OHB, 43 µmol/L, 61%, and 1.5; for lactate, 0.88 mmol/L, 31%, and 1.1; for glucose, 4.9 mmol/L, 4.8%, and 0.7; for insulin, 52 pmol/L, 26%, and 1.0; for C-peptide, 0.39 nmol/L, 24%, and 0.9; and for glucagon, 53 ng/L, 19%, and 0.8.

Conclusions: The data presented here are necessary for the evaluation of several important metabolic variables in individual and group studies. The biological variation of some metabolites makes it difficult to characterize the status of healthy subjects with a single measurement.© 1999 American Association for Clinical Chemistry




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