Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 45: 1070-1076, 1999;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Correction
Right arrow A correction has been published
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hindmarsh, J. T.
Right arrow Articles by Greenway, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hindmarsh, J. T.
Right arrow Articles by Greenway, D. C.
Related Collections
Right arrow Molecular Diagnostics and Genetics
Right arrow Endocrinology and Metabolism
(Clinical Chemistry. 1999;45:1070-1076.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Plasma Porphyrins in the Porphyrias

J. Thomas Hindmarsha, Linda Oliveras and Donald C. Greenway

Division of Biochemistry, Ottawa Hospital, General Campus, 501 Smyth Rd., Ottawa, Ontario, Canada K1H 8L6.
a Author for correspondence. Fax 613 737 8315; e-mail jhindmarsh{at}ogh.on.ca

Background: As an aid in the diagnosis and management of porphyria we have developed a method to fractionate and quantify plasma porphyrins and have evaluated its use in various porphyrias.

Methods: We used HPLC with fluorometric detection to measure plasma concentrations of uroporphyrin I and III, heptacarboxyl III, hexacarboxyl III, pentacarboxyl III, and coproporphyrin I and III. We studied 245 healthy subjects, 32 patients with classical porphyria cutanea tarda (PCT), 12 patients with PCT of renal failure, 13 patients with renal failure, 8 patients with pseudoporphyria of renal failure, 3 patients with acute intermittent porphyria, 5 patients with variegate porphyria, 5 patients with hereditary coproporphyria, and 4 patients with erythropoietic protoporphyria.

Results: Between-run CVs were 5.4–13%. The recoveries of porphyrins added to plasma were 71–114% except for protoporphyrin, which could not be reliably measured with this technique. Plasma porphyrin patterns clearly identified PCT, and its clinical sensitivity equaled that of urine porphyrin fractionation. The patterns also allowed differentiation of PCT of renal failure from pseudoporphyria of renal failure.

Conclusions: The assay of plasma porphyrins identifies patients with PCT and appears particularly useful for differentiating PCT of renal failure from pseudoporphyria of renal failure.© 1999 American Association for Clinical Chemistry




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. PsychiatryHome page
H. L. Gonzalez-Arriaza and J. M. Bostwick
Acute Porphyrias: A Case Report and Review
Am J Psychiatry, March 1, 2003; 160(3): 450 - 459.
[Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
A C Deacon and G H Elder
ACP Best Practice No 165: Front line tests for the investigation of suspected porphyria
J. Clin. Pathol., July 1, 2001; 54(7): 500 - 507.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. A. Pierach
Porphyria Conundrum
Clin. Chem., July 1, 1999; 45(7): 932 - 933.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association for Clinical Chemistry.