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Increased Ability of LDL from Normolipidemic Type 2 Diabetic Women to Generate Peroxides

¹ Service de Diabétologie, Nutrition et Maladies Métaboliques, Centre Hospitalo-Universitaire de Nancy-Hôpital Jeanne d'Arc, 54201 Toul cedex B.P. 303, France.

² Département de Biochimie, UFR des Saints-Pères, Faculté de Médecine Paris-Ouest, Université René Descartes, 75006 Paris, France.

³ Clinique Médicale, Unité 62, Hôpital Robert Debré, Centre Hospitalo-Universitaire de Reims, Rue Alexis Carrel, 51100 Reims, France.

⁴ Biochimie A, Centre Hospitalo-Universitaire de Nancy-Hôpital de Brabois, 54500 Vandoeuvre-Les-Nancy, France.
^a Address correspondence to this author at: Service de Diabétologie, Maladies Métaboliques et Maladies de la Nutrition, Centre d'Investigation Clinique C.I.C.-INSERM/CHU de NANCY, Hôpital Jeanne d'Arc, B.P. 303, 54201 Toul cedex, France. Fax 33-3-83-65-66-00; e-mail cic{at}chu.nancy.fr

Background: We assessed the ability of LDL from 30 type 1 diabetic patients (18 men, 12 women), 65 type 2 diabetic patients (35 men, 30 women), and 35 controls (19 men, 16 women) to generate peroxides. The men and women in the diabetic groups were studied separately and matched for age, body mass index, duration of diabetes, glycohemoglobin, and conventional lipid characteristics according to the presence or absence of hyperlipidemia.

Methods: The ability of LDL to form peroxides was assessed by measuring the thiobarbituric acid-reactive substances corrected for LDL-cholesterol [ratio of malondialdehyde (MDA) to LDL-cholesterol]. LDL particle size was expressed as the ratio of LDL-cholesterol to apolipoprotein B (LDL-cholesterol/apoB).

Results: The MDA/LDL-cholesterol ratio was higher in type 1 and type 2 diabetic patients with hyperlipidemia than in controls. The MDA/LDL-cholesterol ratio was also higher in type 2 normolipidemic women than in controls (P <0.01). The LDL-cholesterol/apoB ratio was lower in type 2 diabetic women than in type 2 diabetic men (P <0.05). The MDA/LDL-cholesterol ratio was negatively correlated with the LDL-cholesterol/apoB ratio (r = -0.78, P <0.001) in hyperlipidemic type 1 (not type 2) diabetic patients. In normolipidemic type 2 diabetic patients, the MDA/LDL-cholesterol ratio was also negatively correlated with the LDL-cholesterol/apoB ratio (r = -0.75, P <0.001) because of the highly significant negative correlation in type 2 diabetic women (r = -0.89, P <0.01).

Conclusions: LDL from well-controlled type 2 diabetic women is smaller and more prone to form peroxides. This could explain why diabetic women are at greater risk of cardiovascular disease.

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				D. Furuya, A. Yagihashi, S. Nasu, T. Endoh, T. Nakamura, R. Kaneko, C. Kamagata, D. Kobayashi, and N. Watanabe LDL Particle Size by Gradient-Gel Electrophoresis Cannot Be Estimated by LDL-Cholesterol/Apolipoprotein B Ratios Clin. Chem., August 1, 2000; 46(8): 1202 - 1203. [Full Text] [PDF]