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Clinical Chemistry 45: 1459-1464, 1999;
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(Clinical Chemistry. 1999;45:1459-1464.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Simple Method for Determination of Thiocyanate in Urine

M. Rezaul Haque and J. Howard Bradburya

a Author for correspondence. Fax 61-2-62495773; e-mail Howard.Bradbury{at}anu.edu.au

Background: It would be useful to develop a simple kit method for determination of thiocyanate in urine, which could be used to monitor cyanide overload in cassava-consuming populations.

Methods: The method was based on the quantitative oxidation of thiocyanate in acid permanganate at room temperature in a closed vial with liberation of HCN, which reacted with a picrate paper. For semiquantitative analysis in the field, the colored picrate paper was matched with a color chart prepared using known amounts of KSCN. In the laboratory, a more accurate result was obtained by elution of the colored complex in water and measurement of the absorbance at 510 nm. Over the range 0–100 mg/L, there was a linear relationship given by the equation: thiocyanate content (mg/L) = 78 x absorbance.

Results: The picrate thiocyanate method gave no interference with urine samples containing protein at less than 7 g/L, 21 amino acids, histamine, glucose, NaCl, urea, blood, and linamarin. For 53 urine samples analyzed by an accurate column method and the thiocyanate picrate method, a regression line gave very good agreement (r2 = 1.000). Quantitative recoveries of thiocyanate added to urine samples were obtained with the picrate method.

Conclusions: A simple picrate kit for determination of thiocyanate in urine was developed and is available free of charge for workers in developing countries.




The following articles in journals at HighWire Press have cited this article:


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 Hum Exp ToxicolHome page
L Rongzhu, W Suhua, X Guangwei, H Fangan, C Ziqiang, J Fusheng, and S Kacew
Neurobehavioral alterations in rats exposed to acrylonitrile in drinking water
Human and Experimental Toxicology, March 1, 2007; 26(3): 179 - 184.
[Abstract] [PDF]


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