Clinical Chemistry
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Clinical Chemistry 45: 1510-1516, 1999;
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(Clinical Chemistry. 1999;45:1510-1516.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Total and Free Deoxypyridinoline after Acute Osteoclast Activity Inhibition

Alessandro Rubinacci1,a, Raffaella Melzi1, Maria Zampino1, Armando Soldarini2 and Isabella Villa1

1 Bone Metabolic Unit and
2 Department of Clinical Chemistry, Scientific Institute H San Raffaele, Via Olgettina 60, 20132 Milan, Italy.
a Address correspondence to this author at: Unita' Metabolica dell' Osso, Istituto Scientifico San Raffaele, Via Olgettina 60, I-20132 Milan, Italy. Fax 39-02-26433038; e-mail a.rubinacci{at}hsr.it

Background: Deoxypyridinoline (Dpd) is one of the two pyridinium cross-links that provide structural rigidity to type I collagen in bone. During osteoclastic resorption, Dpd is released into circulation and is excreted in the urine in free and peptide-bound forms. Free and total Dpd are highly correlated, but whether the free-to-total cross-link ratio is constant in both normal and high bone turnover states remains controversial. To compare free and total Dpd performance in a physiological condition, urinary free and total Dpd were measured after a short-term inhibition of osteoclast activity such as that induced by an oral calcium load.

Methods: Total and free Dpd were measured by HPLC and by immunosorbent assay, respectively, in two groups of subjects, one (calcium-treated; n = 16) taking calcium and the other not (control; n = 9).

Results: The urinary excretion of total Dpd at 2 and 4 h after oral calcium loading was decreased compared with controls. By contrast, changes in free Dpd were similar in the calcium-treated and control groups, reflecting only circadian rhythm.

Conclusions: Total and free Dpd do not show comparable sensitivity in detecting short-term inhibition of osteoclast activity. The degradation process of peptide-bound to free Dpd could render free Dpd insensitive to acute changes of osteoclast activity.




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Does it make a difference how and when you take your calcium? The acute effects of calcium on calcium and bone metabolism
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