Clinical Chemistry
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Clinical Chemistry 45: 1557-1561, 1999;
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(Clinical Chemistry. 1999;45:1557-1561.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Evaluation of a Particle-enhanced Turbidimetric Immunoassay for the Measurement of Immunoglobulin E in an ILab 900 Analyzer

Sandra Guaita, Josep MA Simó, Natàlia Ferré, Jorge Joven and Jordi Campsa

Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Carrer Sant Joan s/n, 43201-Reus, Catalunya, Spain.
a Author for correspondence. Fax 34-77-31-25-69;

Background: The measurement of immunoglobulin E (IgE) in serum is widely used in the diagnosis of allergic reactions and parasitic infections. We describe here a fully automated assay for human IgE suitable for routine application in a general chemistry analyzer.

Methods: We used an ILab 900® analyzer. This instrument automates a particle-enhanced immunoturbidimetric assay with an analysis time of 9 min.

Results: The assay was linear in the range 4–1000 kIU/L (r = 0.9998). The intra- and interassay CVs at 57, 235, and 434 kIU/L were <3.5% and <7.4%, respectively. The detection limit was 4 kIU/L. Hemoglobin (<=16 g/L), bilirubin (<=250 µmol/L), and myeloma paraproteins did not interfere with the assay. The assay showed good correlation with a microparticle enzyme immunoassay (r = 0.998) with a mean difference between methods of -6 ± 26 kIU/L.

Conclusion: The new automated serum assay for IgE is an attractive alternative that avoids the need for dedicated instrumentation.






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Copyright © 1999 by the American Association for Clinical Chemistry.