Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 45: 1628-1650, 1999;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (273)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jayasena, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jayasena, S. D.
Related Collections
Right arrow Oak Ridge Conference
(Clinical Chemistry. 1999;45:1628-1650.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics

Sumedha D. Jayasena

NeXstar Pharmaceuticals, Inc., 2860 Wilderness Place, Boulder, CO 80301. Fax 303-444-0672; e-mail sumedha{at}nexstar.com

Antibodies, the most popular class of molecules providing molecular recognition needs for a wide range of applications, have been around for more than three decades. As a result, antibodies have made substantial contributions toward the advancement of diagnostic assays and have become indispensable in most diagnostic tests that are used routinely in clinics today. The development of the systematic evolution of ligands by exponential enrichment (SELEX) process, however, made possible the isolation of oligonucleotide sequences with the capacity to recognize virtually any class of target molecules with high affinity and specificity. These oligonucleotide sequences, referred to as "aptamers", are beginning to emerge as a class of molecules that rival antibodies in both therapeutic and diagnostic applications. Aptamers are different from antibodies, yet they mimic properties of antibodies in a variety of diagnostic formats. The demand for diagnostic assays to assist in the management of existing and emerging diseases is increasing, and aptamers could potentially fulfill molecular recognition needs in those assays. Compared with the bellwether antibody technology, aptamer research is still in its infancy, but it is progressing at a fast pace. The potential of aptamers may be realized in the near future in the form of aptamer-based diagnostic products in the market. In such products, aptamers may play a key role either in conjunction with, or in place of, antibodies. It is also likely that existing diagnostic formats may change according to the need to better harness the unique properties of aptamers.




The following articles in journals at HighWire Press have cited this article:


Home page
 Antimicrob. Agents Chemother.Home page
P. Bellecave, C. Cazenave, J. Rumi, C. Staedel, O. Cosnefroy, M.-L. Andreola, M. Ventura, L. Tarrago-Litvak, and T. Astier-Gin
Inhibition of Hepatitis C Virus (HCV) RNA Polymerase by DNA Aptamers: Mechanism of Inhibition of In Vitro RNA Synthesis and Effect on HCV-Infected Cells
Antimicrob. Agents Chemother., June 1, 2008; 52(6): 2097 - 2110.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. J. Boese and R. R. Breaker
In vitro selection and characterization of cellulose-binding DNA aptamers
Nucleic Acids Res., October 8, 2007; 35(19): 6378 - 6388.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
Y.-Y. Li, C. Zhang, B.-S. Li, L.-F. Zhao, X.-b. Li, W.-J. Yang, and S.-Q. Xu
Ultrasensitive Densitometry Detection of Cytokines with Nanoparticle-Modified Aptamers
Clin. Chem., June 1, 2007; 53(6): 1061 - 1066.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Lin, J. Yan, Z. Huang, C. Altier, M. Li, N. Carrasco, M. Suyemoto, L. Johnston, S. Wang, Q. Wang, et al.
Design and synthesis of boronic-acid-labeled thymidine triphosphate for incorporation into DNA
Nucleic Acids Res., February 28, 2007; 35(4): 1222 - 1229.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. N. Win, J. S. Klein, and C. D. Smolke
Codeine-binding RNA aptamers and rapid determination of their binding constants using a direct coupling surface plasmon resonance assay
Nucleic Acids Res., November 14, 2006; 34(19): 5670 - 5682.
[Abstract] [Full Text] [PDF]

Home page
 J Biomol ScreenHome page
P. Porschewski, M. A.-M. Grattinger, K. Klenzke, A. Erpenbach, M. R. Blind, and F. Schafer
Using Aptamers as Capture Reagents in Bead-Based Assay Systems for Diagnostics and Hit Identification
J Biomol Screen, October 1, 2006; 11(7): 773 - 781.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Shangguan, Y. Li, Z. Tang, Z. C. Cao, H. W. Chen, P. Mallikaratchy, K. Sefah, C. J. Yang, and W. Tan
From the Cover: Aptamers evolved from live cells as effective molecular probes for cancer study
PNAS, August 8, 2006; 103(32): 11838 - 11843.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Inoue, N. Minakawa, and A. Matsuda
Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA
Nucleic Acids Res., July 19, 2006; 34(12): 3476 - 3483.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. C. Chu, J. W. Marks III, L. A. Lavery, S. Faulkner, M. G. Rosenblum, A. D. Ellington, and M. Levy
Aptamer:Toxin Conjugates that Specifically Target Prostate Tumor Cells.
Cancer Res., June 15, 2006; 66(12): 5989 - 5992.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Kato, N. Minakawa, Y. Komatsu, H. Kamiya, N. Ogawa, H. Harashima, and A. Matsuda
New NTP analogs: the synthesis of 4'-thioUTP and 4'-thioCTP and their utility for SELEX
Nucleic Acids Res., May 24, 2005; 33(9): 2942 - 2951.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. A. Di Giusto, W. A. Wlassoff, J. J. Gooding, B. A. Messerle, and G. C. King
Proximity extension of circular DNA aptamers with real-time protein detection
Nucleic Acids Res., April 7, 2005; 33(6): e64 - e64.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Dellaire, R. Nisman, C. H. Eskiw, and D. P. Bazett-Jones
In situ imaging and isolation of proteins using dsDNA oligonucleotides
Nucleic Acids Res., November 23, 2004; 32(20): e165 - e165.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. A. Di Giusto and G. C. King
Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers
J. Biol. Chem., November 5, 2004; 279(45): 46483 - 46489.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. S. Schmidt, S. Borkowski, J. Kurreck, A. W. Stephens, R. Bald, M. Hecht, M. Friebe, L. Dinkelborg, and V. A. Erdmann
Application of locked nucleic acids to improve aptamer in vivo stability and targeting function
Nucleic Acids Res., October 27, 2004; 32(19): 5757 - 5765.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Weill, D. Louis, and B. Sargueil
Selection and evolution of NTP-specific aptamers
Nucleic Acids Res., September 27, 2004; 32(17): 5045 - 5058.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Hoshika, N. Minakawa, and A. Matsuda
Synthesis and physical and physiological properties of 4'-thioRNA: application to post-modification of RNA aptamer toward NF-{kappa}B
Nucleic Acids Res., July 19, 2004; 32(13): 3815 - 3825.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
D. FAULHAMMER, B. ESCHGFALLER, S. STARK, P. BURGSTALLER, W. ENGLBERGER, J. ERFURTH, F. KLEINJUNG, J. RUPP, S. D. VULCU, W. SCHRODER, et al.
Biostable aptamers with antagonistic properties to the neuropeptide nociceptin/orphanin FQ
RNA, March 1, 2004; 10(3): 516 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. F. Lee, J. R. Hesselberth, L. A. Meyers, and A. D. Ellington
Aptamer Database
Nucleic Acids Res., January 1, 2004; 32(90001): D95 - 100.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Rajendran and A. D. Ellington
In vitro selection of molecular beacons
Nucleic Acids Res., October 1, 2003; 31(19): 5700 - 5713.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. Pileur, M.-L. Andreola, E. Dausse, J. Michel, S. Moreau, H. Yamada, S. A. Gaidamakov, R. J. Crouch, J.-J. Toulme, and C. Cazenave
Selective inhibitory DNA aptamers of the human RNase H1
Nucleic Acids Res., October 1, 2003; 31(19): 5776 - 5788.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
B Tavitian
In vivo imaging with oligonucleotides for diagnosis and drug development
Gut, June 1, 2003; 52(90004): iv40 - 47.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. K. SILVERMAN
Rube Goldberg goes (ribo)nuclear? Molecular switches and sensors made from RNA
RNA, April 1, 2003; 9(4): 377 - 383.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
O. Larsson, D. Thormeyer, A. Asinger, B. Wihlen, C. Wahlestedt, and Z. Liang
Quantitative codon optimisation of DNA libraries encoding sub-random peptides: design and characterisation of a novel library encoding transmembrane domain peptides
Nucleic Acids Res., December 1, 2002; 30(23): e133 - e133.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. C. Cox, A. Hayhurst, J. Hesselberth, T. S. Bayer, G. Georgiou, and A. D. Ellington
Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer
Nucleic Acids Res., October 15, 2002; 30(20): e108 - e108.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
D. Zichi, T. Koga, C. Greef, R. Ostroff, and H. Petach
Photoaptamer Technology: Development of Multiplexed Microarray Protein Assays
Clin. Chem., October 1, 2002; 48(10): 1865 - 1868.
[Full Text] [PDF]

Home page
 Cancer Res.Home page
S. E. Lupold, B. J. Hicke, Y. Lin, and D. S. Coffey
Identification and Characterization of Nuclease-stabilized RNA Molecules That Bind Human Prostate Cancer Cells via the Prostate-specific Membrane Antigen
Cancer Res., July 15, 2002; 62(14): 4029 - 4033.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Ulrich, M. H. Magdesian, M. J. M. Alves, and W. Colli
In Vitro Selection of RNA Aptamers That Bind to Cell Adhesion Receptors of Trypanosoma cruzi and Inhibit Cell Invasion
J. Biol. Chem., May 31, 2002; 277(23): 20756 - 20762.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Srisawat, I. J. Goldstein, and D. R. Engelke
Sephadex-binding RNA ligands: rapid affinity purification of RNA from complex RNA mixtures
Nucleic Acids Res., January 15, 2001; 29(2): e4 - e4.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Capaldi, R. C. Getts, and S. D. Jayasena
Signal amplification through nucleotide extension and excision on a dendritic DNA platform
Nucleic Acids Res., April 1, 2000; 28(7): e21 - e21.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
T. Hermann and D. J. Patel
Adaptive Recognition by Nucleic Acid Aptamers
Science, February 4, 2000; 287(5454): 820 - 825.
[Abstract] [Full Text]

Home page
 J. Clin. Pathol.Home page
R Swaminathan and M Wheeler
Robotics into the millennium
J. Clin. Pathol., January 1, 2000; 53(1): 22 - 26.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Blank, T. Weinschenk, M. Priemer, and H. Schluesener
Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels. SELECTIVE TARGETING OF ENDOTHELIAL REGULATORY PROTEIN PIGPEN
J. Biol. Chem., May 4, 2001; 276(19): 16464 - 16468.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association for Clinical Chemistry.