Clinical Chemistry Siemens Point of Care - Urinalysis
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 45: 1665-1673, 1999;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collison, M. E.
Right arrow Articles by Hoegh, T. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collison, M. E.
Right arrow Articles by Hoegh, T. B.
Related Collections
Right arrow Oak Ridge Conference
Right arrow Endocrinology and Metabolism
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 1999;45:1665-1673.)
© 1999 American Association for Clinical Chemistry, Inc.

Articles

Analytical Characterization of Electrochemical Biosensor Test Strips for Measurement of Glucose in Low-Volume Interstitial Fluid Samples

Michael E. Collison, Philip J. Stouta, Tatyana S. Glushko, Kristen N. Pokela, Debra J. Mullins-Hirte, Joel R. Racchini, Melissa A. Walter, Steve P. Mecca, Joanna Rundquist, John J. Allen, Michael E. Hilgers and Thomas B. Hoegh

Integ, Inc., 2800 Patton Rd., St. Paul, MN 55113.
a Author for correspondence. Fax 651-639-9042; e-mail phil.stout{at}integ-inc.com

Background: Minimally invasive interstitial fluid (ISF) sampling and glucose measurement technologies were integrated into a hand-held device for diabetic glucose monitoring investigations.

Methods: Conventional electrochemical test strip technology (Bayer Glucometer Elite®) was adapted to measure glucose in small (0.5–2.0 µL) samples of ISF. Test strip glucose measurements were performed on a commercial potentiostat and were compared to various reference glucose methodologies (YSI 2300 analyzer, microhexokinase procedure, Bayer Glucometer Elite). Characterizations of the integrated ISF sampling-glucose test strip design included accuracy and precision in various sample media (saline, ISF surrogates, diabetic ISF samples), sample volume dependence, test strip sterilization studies (electron beam, {gamma} irradiation), and diabetic ISF sampling and glucose measurements.

Results: Glucose measurements were free from significant media effects. Sample volume variations (0.6–3.2 µL) revealed only modest dependence of glucose measurement bias on sample volume (-1.5% per microliter). Sterilization treatments had only a minor impact on glucose response and test strip aging and no significant impact on interferent responses of the glucose test strips. Diabetic subject testing under minimum fasting conditions of at least 2 h with integrated ISF sampling and glucose measurement gave low ISF glucose measurement imprecision (CV, 4%) and mean glucose results that were indistinguishable from reference (microhexokinase) ISF glucose measurements and from capillary blood glucose measurements (Glucometer Elite).

Conclusions: Conventional single-use, electrochemical glucose test strip and ISF collection technologies can be readily integrated to provide real-time ISF sampling and glucose measurements for diabetic monitoring applications.




The following articles in journals at HighWire Press have cited this article:


Home page
 J Clin PharmacolHome page
S. Schmidt, R. Banks, V. Kumar, K. H. Rand, and H. Derendorf
Clinical Microdialysis in Skin and Soft Tissues: An Update
J. Clin. Pharmacol., March 1, 2008; 48(3): 351 - 364.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Association for Clinical Chemistry.