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Articles |
Departments of
1
Urology,
2
Laboratory Medicine and Pathobiochemistry, and
3
Pathology, University Hospital Charité, Humboldt University, Schumannstrasse 20/21, D-10098 Berlin, Germany.
a Author for correspondence. Fax 49-30-2802-1402; e-mail klaus.jung{at}charite.de
Background: Patients with prostate cancer (PCa) show a lower ratio of free prostate-specific antigen (fPSA) to total PSA (tPSA) in serum than patients with benign prostatic hyperplasia (BPH). The patterns of the intracellular PSA isoforms in malignant and benign prostatic tissue have been studied as potential molecular reasons for this phenomenon.
Methods: Prostatic tissue samples were obtained after
cystoprostatectomy from patients with bladder cancer (n = 10),
from BPH patients (transurethral resection of the prostate, n =
10; adenomectomy, n = 10), and from the cancerous and noncancerous
parts of the same prostates removed surgically by prostatectomy because
of PCa (n = 20). PSA pattern was characterized by gel filtration,
immunoblotting, and immunoassays for tPSA, fPSA,
1-antichymotrypsin-PSA (ACT-PSA), and complexed PSA
(Bayer Immuno 1 assay). Comparisons were made with the PSA
concentrations in serum.
Results: The major portion of tPSA in all tissue samples was fPSA; complexed PSA forms were <2%. Samples from cystoprostatectomy patients had the lowest and those from adenomectomy patients the highest values of tPSA and fPSA. PSA concentrations were lower in cancerous than in the noncancerous parts of the prostate. No significant correlations were found between tumor stage or grade and the amounts of tPSA, fPSA, and ACT-PSA in tissue. Tissue PSA values were not correlated with the serum PSA concentrations nor with the ratios fPSA/tPSA and ACT-PSA/tPSA in sera.
Conclusions: The amounts of tPSA and the PSA isoforms in prostatic tissue explain neither the concentrations of tPSA and PSA isoforms in serum nor the behavior of the ratio fPSA/tPSA in patients with BPH and PCa.
The following articles in journals at HighWire Press have cited this article:
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  B. BICKERS and C. AUKIM-HASTIE New Molecular Biomarkers for the Prognosis and Management of Prostate Cancer - The Post PSA Era Anticancer Res, August 1, 2009; 29(8): 3289 - 3298. [Abstract] [Full Text] [PDF]
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 J. R. Sterbis, C. Gao, B. Furusato, Y. Chen, S. Shaheduzzaman, L. Ravindranath, D. J. Osborn, I. L. Rosner, A. Dobi, D. G. McLeod, et al. Higher Expression of the Androgen-Regulated Gene PSA/HK3 mRNA in Prostate Cancer Tissues Predicts Biochemical Recurrence-Free Survival Clin. Cancer Res., February 1, 2008; 14(3): 758 - 763. [Abstract] [Full Text] [PDF]
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 A. W. Roddam, C. P. Price, N. E. Allen, and A. M. Ward Assessing the Clinical Impact of Prostate-Specific Antigen Assay Variability and Nonequimolarity: A Simulation Study Based on the Population of the United Kingdom Clin. Chem., June 1, 2004; 50(6): 1012 - 1016. [Abstract] [Full Text] [PDF]
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S. D. Mikolajczyk, W. J. Catalona, C. L. Evans, H. J. Linton, L. S. Millar, K. M. Marker, D. Katir, A. Amirkhan, and H. G. Rittenhouse Proenzyme Forms of Prostate-Specific Antigen in Serum Improve the Detection of Prostate Cancer Clin. Chem., June 1, 2004; 50(6): 1017 - 1025. [Abstract] [Full Text] [PDF]
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 W. A. Colburn Biomarkers in Drug Discovery and Development: From Target Identification through Drug Marketing J. Clin. Pharmacol., April 1, 2003; 43(4): 329 - 341. [Abstract] [Full Text] [PDF]
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C. V. Obiezu, A. Soosaipillai, K. Jung, C. Stephan, A. Scorilas, D. H. C. Howarth, and E. P. Diamandis Detection of Human Kallikrein 4 in Healthy and Cancerous Prostatic Tissues by Immunofluorometry and Immunohistochemistry Clin. Chem., August 1, 2002; 48(8): 1232 - 1240. [Abstract] [Full Text] [PDF]
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L. H. Cazares, B.-L. Adam, M. D. Ward, S. Nasim, P. F. Schellhammer, O. J. Semmes, and G. L. Wright Jr. Normal, Benign, Preneoplastic, and Malignant Prostate Cells Have Distinct Protein Expression Profiles Resolved by Surface Enhanced Laser Desorption/Ionization Mass Spectrometry Clin. Cancer Res., August 1, 2002; 8(8): 2541 - 2552. [Abstract] [Full Text] [PDF]
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 M. A. Serdar, O. Oguz, A. Olgun, B. Seckin, S. Ilgan, A. Hasimi, M. Salih, F. Peker, and T. Kutluay Diagnostic Approach to Prostate Cancer using Total Prostate Specific Antigen-Based Parameters Together Ann. Clin. Lab. Sci., January 1, 2002; 32(1): 22 - 30. [Abstract] [Full Text] [PDF]
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R. Wiese, Y. Belosludtsev, T. Powdrill, P. Thompson, and M. Hogan Simultaneous Multianalyte ELISA Performed on a Microarray Platform Clin. Chem., August 1, 2001; 47(8): 1451 - 1457. [Abstract] [Full Text] [PDF]
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 C. Stephan, K. Jung, M. Lein, P. Sinha, D. Schnorr, and S. A. Loening Molecular Forms of Prostate-specific Antigen and Human Kallikrein 2 as Promising Tools for Early Diagnosis of Prostate Cancer Cancer Epidemiol. Biomarkers Prev., November 1, 2000; 9(11): 1133 - 1147. [Abstract] [Full Text]
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P. Nurmikko, V. Vaisanen, T. Piironen, S. Lindgren, H. Lilja, and K. Pettersson Production and Characterization of Novel Anti-Prostate-specific Antigen (PSA) Monoclonal Antibodies That Do Not Detect Internally Cleaved Lys145-Lys146 Inactive PSA Clin. Chem., October 1, 2000; 46(10): 1610 - 1618. [Abstract] [Full Text] [PDF]
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