| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
1 Departments of Pediatrics, Biochemistry and Molecular Biology, Medical University of South Carolina, 171 Ashley Ave., Charleston, SC 29425. Fax 843-792-1844; e-mail Hollisb{at}musc.edu
Background: Measurement of circulating 25-hydroxyvitamin D [25(OH)D] is important in the management of metabolic bone disease. The aim of this study was to compare two widely used methods for the quantification of circulating 25(OH)D with attention to their abilities to measure 25-hydroxylated ergocalciferol (vitamin D2) [25(OH)D2] and cholecalciferol (vitamin D3) [25(OH)D3].
Methods: We used two commercially available, Food and Drug Administration-approved, radioiodine (125I)-based RIA kits for the detection of 25(OH)D (DiaSorin, Stillwater, MN and IDS Ltd, Tyne and Wear, United Kingdom). These methods were tested for general assay performance, including antibody specificity. Results were compared with those of an HPLC-based direct ultraviolet detection method.
Results: Within- and between-run CVs were 
10%. Both methods
quantitatively recovered 25(OH)D3 added to serum, but only
the DiaSorin kit quantitatively recovered 25(OH)D2. The
primary antibody in the IDS kit had unequal reactivities with pure
25(OH)D2 and 25(OH)D3, whereas the DiaSorin
primary antibody reacted with them equally. In 50 patient samples
assayed by HPLC, the IDS method, but not the DiaSorin method,
underestimated total circulating 25(OH)D when significant circulating
25(OH)D2 was present in patient samples.
Conclusions: Some immunoassays may underestimate total 25(OH)D when 25(OH)D2 constitutes an appreciable part of the total.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  P. Leventis and S. Patel Clinical aspects of vitamin D in the management of rheumatoid arthritis Rheumatology, November 1, 2008; 47(11): 1617 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Jones and P. J. Twomey Issues with vitamin D in routine clinical practice Rheumatology, September 1, 2008; 47(9): 1267 - 1268. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Misra, D. Pacaud, A. Petryk, P. F. Collett-Solberg, M. Kappy, and on behalf of the Drug and Therapeutics Committee o Vitamin D Deficiency in Children and Its Management: Review of Current Knowledge and Recommendations Pediatrics, August 1, 2008; 122(2): 398 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Rovner and K. O. O'Brien Hypovitaminosis D Among Healthy Children in the United States: A Review of the Current Evidence Arch Pediatr Adolesc Med, June 1, 2008; 162(6): 513 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hypponen, S. Turner, P. Cumberland, C. Power, and I. Gibb Serum 25-Hydroxyvitamin D Measurement in a Large Population Survey with Statistical Harmonization of Assay Variation to an International Standard J. Clin. Endocrinol. Metab., December 1, 2007; 92(12): 4615 - 4622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. L Weng, J. Shults, M. B Leonard, V. A Stallings, and B. S Zemel Risk factors for low serum 25-hydroxyvitamin D concentrations in otherwise healthy children and adolescents Am. J. Clinical Nutrition, July 1, 2007; 86(1): 150 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Lata and M. E. Elliott Patient Assessment in the Diagnosis, Prevention, and Treatment of Osteoporosis Nutr Clin Pract, June 1, 2007; 22(3): 261 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Binkley, R. Novotny, D. Krueger, T. Kawahara, Y. G. Daida, G. Lensmeyer, B. W. Hollis, and M. K. Drezner Low Vitamin D Status despite Abundant Sun Exposure J. Clin. Endocrinol. Metab., June 1, 2007; 92(6): 2130 - 2135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Schwalfenberg Not enough vitamin D: Health consequences for Canadians Can Fam Physician, May 1, 2007; 53(5): 841 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. L. Lensmeyer, D. A. Wiebe, N. Binkley, and M. K. Drezner HPLC Method for 25-Hydroxyvitamin D Measurement: Comparison with Contemporary Assays Clin. Chem., June 1, 2006; 52(6): 1120 - 1126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Maunsell, D. J. Wright, and S. J. Rainbow Routine Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry Assay for Simultaneous Measurement of the 25-Hydroxy Metabolites of Vitamins D2 and D3 Clin. Chem., September 1, 2005; 51(9): 1683 - 1690. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Holick, E. S. Siris, N. Binkley, M. K. Beard, A. Khan, J. T. Katzer, R. A. Petruschke, E. Chen, and A. E. de Papp Prevalence of Vitamin D Inadequacy among Postmenopausal North American Women Receiving Osteoporosis Therapy J. Clin. Endocrinol. Metab., June 1, 2005; 90(6): 3215 - 3224. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Levis, A. Gomez, C. Jimenez, L. Veras, F. Ma, S. Lai, B. Hollis, and B. A. Roos Vitamin D Deficiency and Seasonal Variation in an Adult South Florida Population J. Clin. Endocrinol. Metab., March 1, 2005; 90(3): 1557 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Whiting and M. S. Calvo Dietary Recommendations for Vitamin D: a Critical Need for Functional End Points to Establish an Estimated Average Requirement J. Nutr., February 1, 2005; 135(2): 304 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Souberbielle, V. Fayol, C. Sault, E. Lawson-Body, A. Kahan, and C. Cormier Assay-Specific Decision Limits for Two New Automated Parathyroid Hormone and 25-Hydroxyvitamin D Assays Clin. Chem., February 1, 2005; 51(2): 395 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P Heaney Functional indices of vitamin D status and ramifications of vitamin D deficiency Am. J. Clinical Nutrition, December 1, 2004; 80(6): 1706S - 1709S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. G. Armas, B. W. Hollis, and R. P. Heaney Vitamin D2 Is Much Less Effective than Vitamin D3 in Humans J. Clin. Endocrinol. Metab., November 1, 2004; 89(11): 5387 - 5391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Carter, R. Carter, J. Jones, and J. Berry How Accurate Are Assays for 25-Hydroxyvitamin D? Data from the International Vitamin D External Quality Assessment Scheme Clin. Chem., November 1, 2004; 50(11): 2195 - 2197. [Full Text] [PDF]
|
|
|
|
|
|
N. Binkley, D. Krueger, C. S. Cowgill, L. Plum, E. Lake, K. E. Hansen, H. F. DeLuca, and M. K. Drezner Assay Variation Confounds the Diagnosis of Hypovitaminosis D: A Call for Standardization J. Clin. Endocrinol. Metab., July 1, 2004; 89(7): 3152 - 3157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Turpeinen, U. Hohenthal, and U.-H. Stenman Determination of 25-Hydroxyvitamin D in Serum by HPLC and Immunoassay Clin. Chem., September 1, 2003; 49(9): 1521 - 1524. [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Souberbielle, E. Lawson-Body, B. Hammadi, E. Sarfati, A. Kahan, and C. Cormier The Use in Clinical Practice of Parathyroid Hormone Normative Values Established in Vitamin D-Sufficient Subjects J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3501 - 3504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Souberbielle, E. Lawson-Body, and C. Kindermans Authors' Response: Vitamin D Status and Redefining Serum PTH Reference Range in the Elderly J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 947 - 947. [Full Text] [PDF]
|
|
|
|
|
|
P. Glendenning, S. D. Vasikaran, T. Akamizu, and H. Koshiyama Vitamin D Status and Redefining Serum PTH Reference Range in the Elderly J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 946 - 947. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
