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Saposins A, B, C, and D in Plasma of Patients with Lysosomal Storage Disorders

¹ Lysosomal Diseases Research Unit, Department of Chemical Pathology, Women’s and Children’s Hospital, 72 King William Road, North Adelaide, South Australia 5006, Australia.

² School of Biological Sciences and Medicine, The Flinders University of South Australia, P.O. Box 2100, Adelaide 5001, Australia.

³ Division of Human Genetics, Children’s Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229.

⁴ Biochemistry, Endocrinology and Metabolism Unit, Institute of Child Health (University College London), 30 Guilford St., London WC1N 1EH, UK.
^a Author for correspondence. Fax 61-8-204-7100; e-mail pmeikle{at}medicine.adelaide.edu.au

Background: Early diagnosis of lysosomal storage disorders (LSDs), before the onset of irreversible pathology, will be critical for maximum efficacy of many current and proposed therapies. To search for potential markers of LSDs, we measured saposins A, B, C, and D in patients with these disorders.

Methods: Four time-delayed fluorescence immunoquantification assays were used to measure each of the saposins in plasma from 111 unaffected individuals and 334 LSD-affected individuals, representing 28 different disorders.

Results: Saposin A was increased above the 95th centile of the control population in 59% of LSD patients; saposins B, C, and D were increased in 25%, 61%, and 57%, respectively. Saposins were increased in patients from several LSD groups that in previous studies did not show an increase of lysosome-associated membrane protein-1 (LAMP-1).

Conclusion: Saposins may be useful markers for LSDs when used in conjunction with LAMP-1.

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