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Clinical Chemistry 46: 313-318, 2000;
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(Clinical Chemistry. 2000;46:313-318.)
© 2000 American Association for Clinical Chemistry, Inc.

Articles

Real-Time Quantification of Human Telomerase Reverse Transcriptase mRNA in Tumors and Healthy Tissues

Jacques B. de Kok1,a, Theo J.M. Ruers2, Goos N.P. van Muijen3, Adrie van Bokhoven4, Hans L. Willems1 and Dorine W. Swinkels1

Departments of
1 Clinical Chemistry,
2 Surgery,
3 Pathology, and
4 Urology, University Hospital Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
a Author for correspondence. Fax 31-243541743; e-mail J.dekok{at}ckcl.azn.nl

Background: Expression of the hTERT gene, which codes for the catalytic subunit of telomerase, is associated with malignancy. We recently developed a real-time reverse transcription-PCR assay, based on TaqMan technology, for accurate and reproducible determination of hTERT mRNA expression (Lab Investig 1999;79:911–2). This method may be of interest for molecular tumor diagnostics in tissues and corresponding body fluids, washings, or brushes.

Methods: In this study, we measured hTERT expression in a subset of healthy tissues and tumors to select those tumor types with the best potential for quantification of hTERT in corresponding body fluids. To demonstrate the use of the method in body fluids, we quantified hTERT expression in voided urine of patients with bladder cancer and controls.

Results: Real-time measurement of hTERT expression could discriminate between all healthy and malignant tissue samples from pancreas, lung, esophagus, and bladder, but not for colon tissues. Moreover, in five of nine (55%) urine samples, hTERT could be quantified.

Conclusions: The present study demonstrates that accurate quantitative measurement of hTERT expression has high potential for discrimination between healthy and tumor cells in tissues and urine and supports future measurements in pancreatic fluid, bronchoalveolar lavage fluid, esophageal brushings, and urine or bladder washings.




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Erratum table 1
Jacques de Kok
Clinical Chemistry Online, 13 Mar 2000 [Full text]

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