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Clinical Chemistry 46: 351-364, 2000;
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Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
(Clinical Chemistry. 2000;46:351-364.)
© 2000 American Association for Clinical Chemistry, Inc.

Articles

Estimating the Long-Term Effects of Storage at -70 °C on Cholesterol, Triglyceride, and HDL-Cholesterol Measurements in Stored Sera

Weichung Joe Shih1,1, Paul S. Bachorik2,a, Jo A. Haga3,2, Gary L. Myers4 and Evan A. Stein5

1 Merck Research Laboratories, Rahway, NJ 07065.

2 Departments of Pediatrics and Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287 (retired).

3 Wilford Hall Medical Center, Department of Pathology, Lackland Air Force Base, TX 78236.

4 National Center for Environmental Health, Division of Environmental Health Laboratory Sciences, Centers for Disease Control and Prevention, Atlanta, GA 30341.

5 Medical Research Laboratories, Highland Heights, KY 41076.
a Address correspondence to this author at: c/o D. Bartholomew, 11 Fox Run Rd., Falmouth, ME 04105. Fax: 207-797-8596 (primary) or 410-955-1276 (secondary); e-mail pbach{at}prodigy.net

We estimated the effects of long-term storage at -70 °C on serum total cholesterol, HDL-cholesterol, and triglycerides in specimens that had been stored for up to 7 years. These estimates were made using measurements in serial specimens collected from the placebo control group of the Air Force/Texas Coronary Atherosclerosis Prevention Study over a period of ~5 years. We compared the group means for pairs of serial specimens taken at 6- and 12-month intervals, assuming that (a) a negligible placebo effect occurred between the serial specimen pairs; (b) in the absence of storage effects, the variation in the group means would reflect only normal biological variation and would not materially affect the group means for the serial specimens; (c) any systematic changes in these group means would reflect storage-related changes; and (d) storage-related changes are cumulative, i.e., the overall changes for a given storage period are the sum of the changes during previous storage periods. We observed average decreases of 2.0% per year for total cholesterol over 7 years and 2.8% per year in triglycerides for the first 5 years. HDL-cholesterol decreased by 1.3% per year, but this change was not statistically significant. This approach may be useful for estimating storage-related changes for studies in specimens stored for a period of years and for which stability data may not be available.




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