Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 46: 404-411, 2000;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (52)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bagley, P. J.
Right arrow Articles by Selhub, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bagley, P. J.
Right arrow Articles by Selhub, J.
Related Collections
Right arrow Nutrition
Right arrow Endocrinology and Metabolism
Right arrow Automation and Analytical Techniques
(Clinical Chemistry. 2000;46:404-411.)
© 2000 American Association for Clinical Chemistry, Inc.

Articles

Analysis of Folate Form Distribution by Affinity Followed by Reversed-Phase Chromatography with Electrochemical Detection

Pamela J. Bagleya and Jacob Selhub

a Author for correspondence. Fax 617-556-3166; e-mail pbagley{at}hnrc.tufts.edu

Background: Naturally occurring folates exist in multiple forms, differing in pteridine ring structure and number of glutamate residues. The ability to measure these folate coenzymes in tissues and cells gives important information about in vivo folate metabolism.

Methods: Folates were heat-extracted from biological samples. A two-column HPLC system with four-channel coulometric electrochemical detection was used for analysis. An affinity column was used first to purify folates from the extract. Purified folates were eluted from the affinity column onto a phenyl analytical column, utilizing a switching valve, and folate forms were separated using an acetonitrile gradient.

Results: Folate forms differing in pteridine ring structure and number of glutamate chain residues were identified by retention time and characteristic response across the channels of the detector. Folates were quantified by comparison to an external calibration mixture. Limits of detection for pentaglutamyl folates ranged from 0.21 pmol for tetrahydrofolate to 0.41 pmol for 5-methyltetrahydrofolate. CVs (n = 5) for peaks containing 9–67 pmol of folate were 0.6–6.4% (within day) and 5.2–8.4% (between days). CVs (n = 5) for peaks containing 0.9–3.5 pmol folate were 5.7–16% (within day) and 8.4–13% (between days).

Conclusions: This automated HPLC system allows the simultaneous determination of polyglutamyl forms of folates from biological samples, including tetrahydrofolate, 5-methyltetrahydrofolate, formylated folates, and pteroylglutamate. The low detection limits allow analysis of folate form distribution in human samples such as erythrocytes and lymphocytes.




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Clin. Nutr.Home page
R. D Kalmbach, S. F Choumenkovitch, A. M Troen, R. D'Agostino, P. F Jacques, and J. Selhub
Circulating folic acid in plasma: relation to folic acid fortification
Am. J. Clinical Nutrition, September 1, 2008; 88(3): 763 - 768.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
R. Kalmbach, S. Choumenkovitch, P. Jacques, A. Troen, and J. Selhub
Unmetabolized folic acid in plasma: Effect of folic acid fortification
FASEB J, April 1, 2007; 21(5): A104 - A104.


Home page
 J. Biol. Chem.Home page
T. J. Vickers, G. Orsomando, R. D. de la Garza, D. A. Scott, S. O. Kang, A. D. Hanson, and S. M. Beverley
Biochemical and Genetic Analysis of Methylenetetrahydrofolate Reductase in Leishmania Metabolism and Virulence
J. Biol. Chem., December 15, 2006; 281(50): 38150 - 38158.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
R. Poo-Prieto, D. B. Haytowitz, J. M. Holden, G. Rogers, S. F. Choumenkovitch, P. F. Jacques, and J. Selhub
Use of the Affinity/HPLC Method for Quantitative Estimation of Folic Acid in Enriched Cereal-Grain Products
J. Nutr., December 1, 2006; 136(12): 3079 - 3083.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. C. Anguera, M. S. Field, C. Perry, H. Ghandour, E.-P. Chiang, J. Selhub, B. Shane, and P. J. Stover
Regulation of Folate-mediated One-carbon Metabolism by 10-Formyltetrahydrofolate Dehydrogenase
J. Biol. Chem., July 7, 2006; 281(27): 18335 - 18342.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
H. E Gabriel, J. W Crott, H. Ghandour, G. E Dallal, S.-W. Choi, M. K Keyes, H. Jang, Z. Liu, M. Nadeau, A. Johnston, et al.
Chronic cigarette smoking is associated with diminished folate status, altered folate form distribution, and increased genetic damage in the buccal mucosa of healthy adults.
Am. J. Clinical Nutrition, April 1, 2006; 83(4): 835 - 841.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
A. M. Troen, B. Mitchell, B. Sorensen, M. H. Wener, A. Johnston, B. Wood, J. Selhub, A. McTiernan, Y. Yasui, E. Oral, et al.
Unmetabolized Folic Acid in Plasma Is Associated with Reduced Natural Killer Cell Cytotoxicity among Postmenopausal Women
J. Nutr., January 1, 2006; 136(1): 189 - 194.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Orsomando, R. D. de la Garza, B. J. Green, M. Peng, P. A. Rea, T. J. Ryan, J. F. Gregory III, and A. D. Hanson
Plant {gamma}-Glutamyl Hydrolases and Folate Polyglutamates: CHARACTERIZATION, COMPARTMENTATION, AND CO-OCCURRENCE IN VACUOLES
J. Biol. Chem., August 12, 2005; 280(32): 28877 - 28884.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Goyer, E. Collakova, R. D. de la Garza, E. P. Quinlivan, J. Williamson, J. F. Gregory III, Y. Shachar-Hill, and A. D. Hanson
5-Formyltetrahydrofolate Is an Inhibitory but Well Tolerated Metabolite in Arabidopsis Leaves
J. Biol. Chem., July 15, 2005; 280(28): 26137 - 26142.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. R. Davis, E. P. Quinlivan, K. P. Shelnutt, D. R. Maneval, H. Ghandour, A. Capdevila, B. S. Coats, C. Wagner, J. Selhub, L. B. Bailey, et al.
The Methylenetetrahydrofolate Reductase 677C->T Polymorphism and Dietary Folate Restriction Affect Plasma One-Carbon Metabolites and Red Blood Cell Folate Concentrations and Distribution in Women
J. Nutr., May 1, 2005; 135(5): 1040 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. R. Davis, E. P. Quinlivan, K. P. Shelnutt, H. Ghandour, A. Capdevila, B. S. Coats, C. Wagner, B. Shane, J. Selhub, L. B. Bailey, et al.
Homocysteine Synthesis Is Elevated but Total Remethylation Is Unchanged by the Methylenetetrahydrofolate Reductase 677C->T Polymorphism and by Dietary Folate Restriction in Young Women
J. Nutr., May 1, 2005; 135(5): 1045 - 1050.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
E. P. Quinlivan, S. R. Davis, K. P. Shelnutt, G. N. Henderson, H. Ghandour, B. Shane, J. Selhub, L. B. Bailey, P. W. Stacpoole, and J. F. Gregory III
Methylenetetrahydrofolate Reductase 677C->T Polymorphism and Folate Status Affect One-Carbon Incorporation into Human DNA Deoxynucleosides
J. Nutr., March 1, 2005; 135(3): 389 - 396.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
H. Ghandour, Z. Chen, J. Selhub, and R. Rozen
Mice Deficient in Methylenetetrahydrofolate Reductase Exhibit Tissue-Specific Distribution of Folates
J. Nutr., November 1, 2004; 134(11): 2975 - 2978.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. D. de la Garza, E. P. Quinlivan, S. M. J. Klaus, G. J. C. Basset, J. F. Gregory III, and A. D. Hanson
Folate biofortification in tomatoes by engineering the pteridine branch of folate synthesis
PNAS, September 21, 2004; 101(38): 13720 - 13725.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
G. P. Basten, M. H. Hill, S. J. Duthie, and H. J. Powers
Effect of Folic Acid Supplementation on the Folate Status of Buccal Mucosa and Lymphocytes
Cancer Epidemiol. Biomarkers Prev., July 1, 2004; 13(7): 1244 - 1249.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
T. H. Kim, J. Yang, P. B. Darling, and D. L. O'Connor
A Large Pool of Available Folate Exists in the Large Intestine of Human Infants and Piglets
J. Nutr., June 1, 2004; 134(6): 1389 - 1394.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S.-W. Choi, S. Friso, H. Ghandour, P. J. Bagley, J. Selhub, and J. B. Mason
Vitamin B-12 Deficiency Induces Anomalies of Base Substitution and Methylation in the DNA of Rat Colonic Epithelium
J. Nutr., April 1, 2004; 134(4): 750 - 755.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. M. Pfeiffer, Z. Fazili, L. McCoy, M. Zhang, and E. W. Gunter
Determination of Folate Vitamers in Human Serum by Stable-Isotope-Dilution Tandem Mass Spectrometry and Comparison with Radioassay and Microbiologic Assay
Clin. Chem., February 1, 2004; 50(2): 423 - 432.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
L. Lathrop Stern, B. Shane, P. J. Bagley, M. Nadeau, V. Shih, and J. Selhub
Combined Marginal Folate and Riboflavin Status Affect Homocysteine Methylation in Cultured Immortalized Lymphocytes from Persons Homozygous for the MTHFR C677T Mutation
J. Nutr., September 1, 2003; 133(9): 2716 - 2720.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. C. Anguera, J. R. Suh, H. Ghandour, I. M. Nasrallah, J. Selhub, and P. J. Stover
Methenyltetrahydrofolate Synthetase Regulates Folate Turnover and Accumulation
J. Biol. Chem., August 8, 2003; 278(32): 29856 - 29862.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
D. Girelli, N. Martinelli, F. Pizzolo, S. Friso, O. Olivieri, C. Stranieri, E. Trabetti, G. Faccini, E. Tinazzi, P. F. Pignatti, et al.
The Interaction between MTHFR 677 C->T Genotype and Folate Status Is a Determinant of Coronary Atherosclerosis Risk
J. Nutr., May 1, 2003; 133(5): 1281 - 1285.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S.-W. Choi, S. Friso, G. G. Dolnikowski, P. J. Bagley, A. N. Edmondson, D. E. Smith, and J. B. Mason
Biochemical and Molecular Aberrations in the Rat Colon Due to Folate Depletion Are Age-Specific
J. Nutr., April 1, 2003; 133(4): 1206 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
J. B. Mason
Biomarkers of Nutrient Exposure and Status in One-Carbon (Methyl) Metabolism
J. Nutr., March 1, 2003; 133(3): 941S - 947.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. L. Jones and P. F. Nixon
Tetrahydrofolates Are Greatly Stabilized by Binding to Bovine Milk Folate-Binding Protein
J. Nutr., September 1, 2002; 132(9): 2690 - 2694.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Friso, S.-W. Choi, D. Girelli, J. B. Mason, G. G. Dolnikowski, P. J. Bagley, O. Olivieri, P. F. Jacques, I. H. Rosenberg, R. Corrocher, et al.
A common mutation in the 5,10-methylenetetrahydrofolate reductase gene affects genomic DNA methylation through an interaction with folate status
PNAS, April 16, 2002; 99(8): 5606 - 5611.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Friso, S.-W. Choi, D. Girelli, J. B. Mason, G. G. Dolnikowski, P. J. Bagley, O. Olivieri, P. F. Jacques, I. H. Rosenberg, R. Corrocher, et al.
A common mutation in the 5,10-methylenetetrahydrofolate reductase gene affects genomic DNA methylation through an interaction with folate status
PNAS, April 16, 2002; 99(8): 5606 - 5611.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Association for Clinical Chemistry.