Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 46: 461-468, 2000;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (114)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roberts, W. L.
Right arrow Articles by Rifai, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roberts, W. L.
Right arrow Articles by Rifai, N.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2000;46:461-468.)
© 2000 American Association for Clinical Chemistry, Inc.

Articles

Evaluation of Four Automated High-Sensitivity C-Reactive Protein Methods: Implications for Clinical and Epidemiological Applications

William L. Roberts1,a, Rachel Sedrick2, Linda Moulton2, Anthony Spencer3 and Nader Rifai3

1 Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, UT 84132.

2 ARUP Institute for Experimental and Clinical Pathology, Salt Lake City, UT 84108.

3 Departments of Laboratory Medicine and Pathology, Children’s Hospital and Harvard Medical School, Boston, MA 02115.
a Address correspondence to this author at: c/o ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108. Fax 801-584-5207; e-mail william.roberts{at}arup-lab.com

Background: C-reactive protein (CRP) can provide prognostic information about the risk of developing atherosclerotic complications in apparently healthy patients. This new clinical application requires quantification of CRP concentrations below those traditionally measured in the clinical laboratory.

Methods: The Dade Behring BN II, the Abbott IMx, the Diagnostic Products Corporation IMMULITE, and the Beckman Coulter IMMAGE are four automated analyzers with high-sensitivity CRP (hs-CRP) methods. We evaluated these assays for precision, linearity, and comparability with samples from 322 apparently healthy blood donors.

Results: The imprecision (CV) of the BN II, IMx, IMMULITE, and IMMAGE methods was <=7.6%, <=12%, <=9.8%, and <=9.7% at 3.5 mg/L, respectively. The BN II, IMx, IMMULITE, and IMMAGE methods were linear down to <=0.30, <=0.32, <=0.85, and 2.26 mg/L, respectively. CRP concentrations demarcating each quartile in a healthy population were method dependent. The IMx method gave results comparable to the BN II method for values in the reference interval. The IMMULITE method had a positive intercept compared with the BN II method. The IMMAGE method demonstrated more scatter and a positive intercept compared with the BN II method, which may reflect the fact that it is a less sensitive assay.

Conclusions: The four hs-CRP methods exhibited differences in results for a healthy population. Additional standardization efforts are required to ensure that hs-CRP results can be related to large-scale epidemiologic studies.




The following articles in journals at HighWire Press have cited this article:


Home page
 IOVSHome page
K. H. C. Wu, A. G. Tan, E. Rochtchina, E. J. Favaloro, A. Williams, P. Mitchell, and J. J. Wang
Circulating Inflammatory Markers and Hemostatic Factors in Age-Related Maculopathy: A Population-Based Case-Control Study
Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 1983 - 1988.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Rifai, C. M. Ballantyne, M. Cushman, D. Levy, and G. L. Myers
Point: High-Sensitivity C-Reactive Protein and Cardiac C-Reactive Protein Assays: Is There a Need to Differentiate?
Clin. Chem., July 1, 2006; 52(7): 1254 - 1256.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. V. Callaghan and S. I. Gutman
Counterpoint: food and drug administration guidance for C-reactive protein assays: matching claims with performance data.
Clin. Chem., July 1, 2006; 52(7): 1256 - 1257.
[Full Text] [PDF]

Home page
 Eur Respir JHome page
M. Takemura, H. Matsumoto, A. Niimi, T. Ueda, H. Matsuoka, M. Yamaguchi, M. Jinnai, S. Muro, T. Hirai, Y. Ito, et al.
High sensitivity C-reactive protein in asthma.
Eur. Respir. J., May 1, 2006; 27(5): 908 - 912.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
D. E. Laaksonen, L. Niskanen, K. Nyyssonen, K. Punnonen, T.-P. Tuomainen, and J. T. Salonen
C-reactive protein in the prediction of cardiovascular and overall mortality in middle-aged men: a population-based cohort study
Eur. Heart J., September 1, 2005; 26(17): 1783 - 1789.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
G. L. Myers, N. Rifai, R. P. Tracy, W. L. Roberts, R. W. Alexander, L. M. Biasucci, J. D. Catravas, T. G. Cole, G. R. Cooper, B. V. Khan, et al.
CDC/AHA Workshop on Markers of Inflammation and Cardiovascular Disease: Application to Clinical and Public Health Practice: Report From the Laboratory Science Discussion Group
Circulation, December 21, 2004; 110(25): e545 - e549.
[Full Text] [PDF]

Home page
 CirculationHome page
W. L. Roberts
CDC/AHA Workshop on Markers of Inflammation and Cardiovascular Disease: Application to Clinical and Public Health Practice: Laboratory Tests Available to Assess Inflammation--Performance and Standardization: A Background Paper
Circulation, December 21, 2004; 110(25): e572 - e576.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. S. Levinson, J. J. Miller, and R. J. Elin
Poor Predictive Value of High-Sensitivity C-Reactive Protein Indicates Need for Reassessment
Clin. Chem., October 1, 2004; 50(10): 1733 - 1735.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. W. McDade, J. Burhop, and J. Dohnal
High-Sensitivity Enzyme Immunoassay for C-Reactive Protein in Dried Blood Spots
Clin. Chem., March 1, 2004; 50(3): 652 - 654.
[Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Artieda, A. Cenarro, A. Ganan, I. Jerico, C. Gonzalvo, J. M. Casado, I. Vitoria, J. Puzo, M. Pocovi, and F. Civeira
Serum Chitotriosidase Activity Is Increased in Subjects With Atherosclerosis Disease
Arterioscler Thromb Vasc Biol, September 1, 2003; 23(9): 1645 - 1652.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. B. Ledue and N. Rifai
Preanalytic and Analytic Sources of Variations in C-reactive Protein Measurement: Implications for Cardiovascular Disease Risk Assessment
Clin. Chem., August 1, 2003; 49(8): 1258 - 1271.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
E. S. Ford, W. H. Giles, G. L. Myers, N. Rifai, P. M. Ridker, and D. M. Mannino
C-reactive Protein Concentration Distribution among US Children and Young Adults: Findings from the National Health and Nutrition Examination Survey, 1999-2000
Clin. Chem., August 1, 2003; 49(8): 1353 - 1357.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. M. Kimberly, H. W. Vesper, S. P. Caudill, G. R. Cooper, N. Rifai, F. Dati, and G. L. Myers
Standardization of Immunoassays for Measurement of High-Sensitivity C-reactive Protein. Phase I: Evaluation of Secondary Reference Materials
Clin. Chem., April 1, 2003; 49(4): 611 - 616.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
T. C. Vukovich, S. Mustafa, H. Rumpold, and O. Wagner
Evaluation of a Turbidimetric Denka Seiken C-Reactive Protein Assay for Cardiovascular Risk Estimation and Conventional Inflammation Diagnosis
Clin. Chem., March 1, 2003; 49(3): 511 - 512.
[Full Text] [PDF]

Home page
 CirculationHome page
T. McLaughlin, F. Abbasi, C. Lamendola, L. Liang, G. Reaven, P. Schaaf, and P. Reaven
Differentiation Between Obesity and Insulin Resistance in the Association With C-Reactive Protein
Circulation, December 3, 2002; 106(23): 2908 - 2912.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Middleton
Effect of Analytical Error on the Assessment of Cardiac Risk by the High-Sensitivity C-Reactive Protein and Lipid Screening Model
Clin. Chem., November 1, 2002; 48(11): 1955 - 1962.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Ishibashi, Y. Takemura, H. Ishida, K. Watanabe, and T. Kawai
C-Reactive Protein Kinetics in Newborns: Application of a High-Sensitivity Analytic Method in Its Determination
Clin. Chem., July 1, 2002; 48(7): 1103 - 1106.
[Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. K.C. Ng, P. Y. Liu, A. J. Williams, S. Nakhla, L. P. Ly, D. J. Handelsman, and D. S. Celermajer
Prospective Study of Effect of Androgens on Serum Inflammatory Markers in Men
Arterioscler Thromb Vasc Biol, July 1, 2002; 22(7): 1136 - 1141.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
S. Liu, J. E Manson, J. E Buring, M. J Stampfer, W. C Willett, and P. M Ridker
Relation between a diet with a high glycemic load and plasma concentrations of high-sensitivity C-reactive protein in middle-aged women
Am. J. Clinical Nutrition, March 1, 2002; 75(3): 492 - 498.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
S. S. Levinson and R. J. Elin
What Is C-Reactive Protein Telling Us About Coronary Artery Disease?
Arch Intern Med, February 25, 2002; 162(4): 389 - 392.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Rothkrantz-Kos, M. P.J. Schmitz, O. Bekers, P. P.C.A. Menheere, and M. P. van Dieijen-Visser
High-Sensitivity C-Reactive Protein Methods Examined
Clin. Chem., February 1, 2002; 48(2): 359 - 362.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Hamwi, T. Vukovich, O. Wagner, H. Rumpold, R. Spies, M. Stich, and C. Langecker
Evaluation of Turbidimetric High-Sensitivity C-Reactive Protein Assays for Cardiovascular Risk Estimation
Clin. Chem., November 1, 2001; 47(11): 2044 - 2046.
[Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
M. Feldman, I. Jialal, S. Devaraj, and B. Cryer
Effects of low-dose aspirin on serum C-reactive protein and thromboxane B2 concentrations: a placebo-controlled study using a highly sensitive C-reactive protein assay
J. Am. Coll. Cardiol., June 15, 2001; 37(8): 2036 - 2041.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
P. M. Ridker
High-Sensitivity C-Reactive Protein : Potential Adjunct for Global Risk Assessment in the Primary Prevention of Cardiovascular Disease
Circulation, April 3, 2001; 103(13): 1813 - 1818.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Rifai and P. M. Ridker
High-Sensitivity C-Reactive Protein: A Novel and Promising Marker of Coronary Heart Disease
Clin. Chem., March 1, 2001; 47(3): 403 - 411.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
W. L. Roberts, L. Moulton, T. C. Law, G. Farrow, M. Cooper-Anderson, J. Savory, and N. Rifai
Evaluation of Nine Automated High-Sensitivity C-Reactive Protein Methods: Implications for Clinical and Epidemiological Applications. Part 2
Clin. Chem., March 1, 2001; 47(3): 418 - 425.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
L. Borque, L. Bellod, A. Rus, M. L. Seco, and F. Galisteo-Gonzalez
Development and Validation of an Automated and Ultrasensitive Immunoturbidimetric Assay for C-Reactive Protein
Clin. Chem., November 1, 2000; 46(11): 1839 - 1842.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Gochman
High and Higher Sensitivity CRP
Clin. Chem., July 1, 2000; 46(7): 1022 - 1022.
[Full Text] [PDF]

eLetters:

Read all eLetters

High Sensitivity CRP
Nathan Gochman
Clinical Chemistry Online, 14 Apr 2000 [Full text]
Response to Dr. Gochman's eLetter
William Roberts
Clinical Chemistry Online, 28 Apr 2000 [Full text]
C-Reactive Protein Reference Limits
Mark Wener
Clinical Chemistry Online, 13 Jul 2000 [Full text]

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Association for Clinical Chemistry.