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Clinical Chemistry 47: 1782-1787, 2001;
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(Clinical Chemistry. 2001;47:1782-1787.)
© 2001 American Association for Clinical Chemistry, Inc.

Articles

Comparison of HPLC and Small Column (CDTect) Methods for Disialotransferrin

Ursula Turpeinen1a, Taina Methuen2, Henrik Alfthan1, Kalevi Laitinen3, Mikko Salaspuro2 and Ulf-Håkan Stenman1

Departments of
1 Clinical Chemistry and
3 Internal Medicine, Helsinki University Central Hospital, 00290 Helsinki, Finland.
2 Research Unit of Alcohol Diseases, University of Helsinki, 00290 Helsinki, Finland.

aAddress correspondence to this author at: Helsinki University Central Hospital, Department of Clinical Chemistry, Haartmaninkatu 2, 00290 Helsinki, Finland. Fax 358-9-471-4945; e-mail ursula.turpeinen{at}hus.fi.

Background: Current methods for determination of carbohydrate-deficient transferrin (CDT) are based on separation of the CDT fraction by ion-exchange chromatography on minicolumns and quantification by immunoassay. Alternatively, the transferrin isoforms can be separated by HPLC anion-exchange chromatography and quantified by absorbance. This method has been reported to improve the validity of CDT as a marker of chronic alcohol abuse.

Methods: HPLC on either MonoQ or ResourceQ anion-exchange columns was used to separate and quantify isoforms of transferrin with detection at 460 nm. The result was expressed as the percentage of the disialo form (pI 5.7) of total transferrin (DST). The commercial CDTectTM assay was used as a comparison method. Serum samples from nondrinkers (n = 57), moderate drinkers (n = 77), and heavy drinkers (n = 139) were analyzed.

Results: In ROC analysis for differentiation between moderate and heavy drinkers, the area under the curve (AUC) for the HPLC method was 0.87 (95% confidence interval, 0.81–0.93), whereas that for CDTect was 0.72 (95% confidence interval, 0.64–0.80). At 90% specificity, the sensitivity of DST was 63% (95% confidence interval, 53–73%) compared with 33% (22–44%) for CDT. The reference interval of the HPLC method was 0.68–1.7%.

Conclusions: The HPLC anion-exchange method for quantification of CDT provides substantially better separation between moderate and heavy drinkers than the CDTect method.




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