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Clinical Chemistry 47: 1829-1835, 2001;
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(Clinical Chemistry. 2001;47:1829-1835.)
© 2001 American Association for Clinical Chemistry, Inc.

Articles

Microvolume Blood-Sampling Device with Low Hemolysis and High Consistent Yield of Serum Components

Yoshiyuki Tanakaa1, Yuichiro Noda1, Mayumi Kobayashi1, Yasuko Yamada1 and Konomu Hirao1

1 Market Development Group, PLUSCARE Division, Arkray Inc., 57 Nishi-aketa-cho, Higashi-kujo, Kyoto, Japan 601-8045.

aAuthor for correspondence. Fax 81-75-662-8961; e-mail tanakayos{at}arkray.co.jp.

Background: Blood sampling by finger puncture is convenient, but the need for centrifugation and the problem of hemolysis remain, as does instability when samples must be shipped for analysis. We aimed to develop a blood-sampling device that provided high yields of serum with limited hemolysis and enabled preservation of serum components for at least 7 days at room temperature.

Methods: For separation of blood cells, we devised a grooved, asymmetric, polysulfonate membrane impregnated with sucrose. We evaluated hemoglobin (Hb) concentrations in the serum, assay values for 15 frequently measured serum components (including glucose), and the stability of analytes in the device.

Results: In sera from the new device, the Hb concentration was <=0.43 mg/L. Recovered serum contained 65.0% ± 4.2% (mean ± SD; n = 41) of each of the serum components obtained by centrifugation. Serum components were stable in the device for 10 days at room temperature (25 °C).

Conclusions: The newly developed device allows recovery of 60% of serum components from microvolumes of blood by finger puncture with neither degradation of analytes at room temperature nor hemolysis.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
G. A. Maguire and C. N. Hales
Use of Sodium Measurement as a Surrogate Volume Measurement in Unmetered-Blood Collection Devices
Clin. Chem., March 1, 2002; 48(3): 592 - 593.
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