HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Hessner, M. J. Articles by Raife, T. J. Search for Related Content PubMed PubMed Citation Articles by Hessner, M. J. Articles by Raife, T. J. Related Collections Molecular Diagnostics and Genetics Oak Ridge Conference

Age-dependent Prevalence of Vascular Disease-associated Polymorphisms among 2689 Volunteer Blood Donors

¹ Max McGee National Research Center for Juvenile Diabetes, The Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226.

² Diagnostic Laboratories, Blood Center of Southeastern Wisconsin, Milwaukee, WI 53201.

³ Third Wave Technologies Inc., Madison, WI 53719.

^aAuthor for correspondence. Fax 414-456-6663; e-mail mhessner{at}mcw.edu.

Abstract

Background: The development of vascular disease involves the interaction of genetic and environmental factors. Because vascular disease is a major contributor to mortality in Western societies, we hypothesized that deleterious polymorphisms associated with hemostasis decrease in frequency among a healthy population as a function of age.

Methods: The frequencies of factor V G1691A Leiden (FVL), factor II (FII) G20210A, methylenetetrahydrofolate reductase (MTHFR) C677T, glycoprotein Ia (GPIa) C807T, glycoprotein IIIa (Pl^A1/Pl^A2) T1565C, and angiotensin-converting enzyme (ACE) intron 16 insertion/deletion (I/D) alleles were determined among 2689 healthy Caucasian whole-blood donors. For analysis, participants were divided into three age groups: 17–39 years (n = 979; 505 males and 474 females), 40–59 years (n = 900; 526 males and 374 females), and 60–85 years (n = 810; 530 males and 280 females).

Results: The Pl^A2 allele frequency decreased from 17.5% to 15.7% and 14.1% in the 17–39 years, 40–59 years, and 60–85 years age groups, respectively (n = 5094 alleles; P = 0.025). Among ACE DD males, the Pl^A2 allele frequency decreased from 20.8% to 16.1% and 9.1% in the same groups, respectively (n = 810 alleles; P = 0.001). No statistically significant decrease in genotype or allele frequency was observed among carriers of FVL, FII 20210A, MTHFR 677T, GPIa 807T, or ACE D.

Conclusions: These data suggest that Pl^A2 carriers, especially those who are ACE DD, are statistically less prevalent among older healthy blood donors compared with their younger counterparts. These observations suggest an important, deleterious, time-dependent impact of the Pl^A2 allele, as well as the ACE DD/Pl^A2 allelic combination, on overall health and longevity.

The following articles in journals at HighWire Press have cited this article:

				J.-K. Yang, Y.-Y. Gong, Liang Xie, S.-G. Lian, Juan Yang, L.-Y. Xu, S.-J. Gao, and Y.-P. Zhang Lack of genetic association between the angiotensin-converting enzyme gene insertion/deletion polymorphism and longevity in a Han Chinese population Journal of Renin-Angiotensin-Aldosterone System, June 1, 2009; 10(2): 115 - 118. [Abstract] [PDF]

				M. D. Fallin and A. Matteini Genetic Epidemiology in Aging Research J Gerontol A Biol Sci Med Sci, January 23, 2009; (2009) gln021v1. [Abstract] [Full Text] [PDF]

				D. Seripa, M. Franceschi, M. G. Matera, F. Panza, P. G. Kehoe, C. Gravina, G. Orsitto, V. Solfrizzi, G. Di Minno, B. Dallapiccola, et al. Sex differences in the association of apolipoprotein e and Angiotensin-converting enzyme gene polymorphisms with healthy aging and longevity: a population-based study from southern Italy. J. Gerontol. A Biol. Sci. Med. Sci., September 1, 2006; 61(9): 918 - 923. [Abstract] [Full Text] [PDF]

				B. Jilma, F. M. Kovar, G. Hron, G. Endler, C. L. Marsik, S. Eichinger, and P. A. Kyrle Homozygosity in the Single Nucleotide Polymorphism Ser128Arg in the E-Selectin Gene Associated With Recurrent Venous Thromboembolism. Arch Intern Med, August 14, 2006; 166(15): 1655 - 1659. [Abstract] [Full Text] [PDF]

				C. J. Glueck, N. Goldenberg, P. Wang, and D. Aregawi Ramifications of Four Concurrent Thrombophilic Mutations and One Hypofibrinolytic Mutation Clinical and Applied Thrombosis/Hemostasis, October 1, 2004; 10(4): 365 - 371. [Abstract] [PDF]

				A. Slowik, T. Dziedzic, W. Turaj, J. Pera, L. Glodzik-Sobanska, P. Szermer, M. T. Malecki, D. A. Figlewicz, and A. Szczudlik A2 Alelle of GpIIIa Gene Is a Risk Factor for Stroke Caused by Large-Vessel Disease in Males Stroke, July 1, 2004; 35(7): 1589 - 1593. [Abstract] [Full Text] [PDF]

				G. H. Gibbons, C. C. Liew, M. O. Goodarzi, J. I. Rotter, W. A. Hsueh, H. M. Siragy, R. Pratt, and V. J. Dzau Genetic Markers: Progress and Potential for Cardiovascular Disease Circulation, June 29, 2004; 109(25_suppl_1): IV-47 - IV-58. [Full Text] [PDF]

				M. Klerk, P. Verhoef, R. Clarke, H. J. Blom, F. J. Kok, E. G. Schouten, and and the MTHFR Studies Collaboration Group MTHFR 677C->T Polymorphism and Risk of Coronary Heart Disease: A Meta-analysis JAMA, October 23, 2002; 288(16): 2023 - 2031. [Abstract] [Full Text] [PDF]