Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 47: 1023-1030, 2001;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fredericks, S.
Right arrow Articles by Holt, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fredericks, S.
Right arrow Articles by Holt, D. W.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2001;47:1023-1030.)
© 2001 American Association for Clinical Chemistry, Inc.

Articles

Cardiac Troponin T and Creatine Kinase MB Are Not Increased in Exterior Oblique Muscle of Patients with Renal Failure

Salim Fredericks1a, Joanne F. Murray2, Michael Bewick3, René Chang3, Paul O. Collinson4, Nicholas D. Carter2 and David W. Holt1

1 Analytical Unit, Cardiological Sciences, and
2 Medical Genetics Unit, St. George’s Hospital Medical School, London SW17 0RE, United Kingdom; and Departments of
3 Renal Medicine and
4 Chemical Pathology, St. George’s Hospital, London SW17 0QT, United Kingdom.

aAuthor for correspondence. Fax 44-20-8767-9687; e-mail frederic{at}sghms.ac.uk.

Background: Serum cardiac troponin T (cTnT) concentrations may be increased in patients with renal dysfunction without evidence of cardiac damage, as assessed by conventional methods. It has been suggested that these positive measurements result from the expression in skeletal muscle of fetal isoforms of cTnT, which are detected by the cTnT immunoassay.

Methods: Skeletal muscle (exterior oblique) biopsies were taken from healthy living kidney donors (n = 5) and transplant recipients (n = 19). The amounts of cTnT and creatine kinase (CK) isoenzymes in skeletal muscle of healthy controls were compared with those in patients with renal failure (Wilcoxon–Mann–Whitney test). cTnT was measured quantitatively by a second-generation assay, with a limit of detection of 1 µg/g of protein, and qualitatively by immunohistochemistry and immunoblotting. CK-MB was measured by quantitative electrophoresis.

Results: Minute quantities of cTnT were detected in 2 of the 5 (40%) control samples and 9 of the 19 (47%) renal failure samples, respectively, at mean concentrations of <5 µg/g of protein for both subject groups. This was <1/6000th that found in heart muscle. There was no significant difference in cTnT or CK-MB content in skeletal muscle between healthy controls and patients with renal failure. Increased serum cTnT did not predict detectable cTnT in skeletal muscle. cTnT was not detected qualitatively by immunoblotting or immunohistochemistry in any skeletal muscle samples.

Conclusions: Uremia does not affect the content of cTnT or CK-MB in exterior oblique muscle, suggesting that cTnT detected in serum from patients with renal failure does not originate from skeletal muscle.




The following articles in journals at HighWire Press have cited this article:


Home page
 Ann Clin BiochemHome page
L. H Jacobs, J. van de Kerkhof, A. M Mingels, V. W Kleijnen, F. M van der Sande, W. K Wodzig, J. P Kooman, and M. P van Dieijen-Visser
Haemodialysis patients longitudinally assessed by highly sensitive cardiac troponin T and commercial cardiac troponin T and cardiac troponin I assays
Ann Clin Biochem, July 1, 2009; 46(4): 283 - 290.
[Abstract] [Full Text] [PDF]

Home page
 Ann Clin BiochemHome page
P. O Collinson and D. C Gaze
Cardiac troponins in patients with renal failure: what are we measuring and when should we measure it?
Ann Clin Biochem, July 1, 2009; 46(4): 269 - 270.
[Full Text] [PDF]

Home page
 HeartHome page
P O Collinson and P J Stubbs
Are troponins confusing?
Heart, November 1, 2003; 89(11): 1285 - 1287.
[Full Text] [PDF]

Home page
 CirculationHome page
C. W. Hamm, E. Giannitsis, and H. A. Katus
Cardiac Troponin Elevations in Patients Without Acute Coronary Syndrome
Circulation, December 3, 2002; 106(23): 2871 - 2872.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Fredericks, J. F. Murray, N. D. Carter, A. M.S. Chesser, S. Papachristou, M. M. Yaqoob, P. O. Collinson, D. Gaze, and D. W. Holt
Cardiac Troponin T and Creatine Kinase MB Content in Skeletal Muscle of the Uremic Rat
Clin. Chem., June 1, 2002; 48(6): 859 - 868.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Association for Clinical Chemistry.