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Reviews |
1 Bio-Analytical Innovations Team, LGC, Queens Road, Teddington, Middlesex, United Kingdom TW11 0LY.
aAuthor for correspondence. Fax 44-20-8943-7335; e-mail caf{at}lgc.co.uk.
Background: Advances in molecular diagnostic technologies have enabled genetic testing in single closed-tube reactions. The purpose of this review is to highlight some of the platforms and technologies currently available for the homogeneous detection of targets and the application of the technologies in the clinical setting. Validation issues surrounding the technologies, which may need to be addressed before they can become widely accepted, will also be discussed.
Approach: This review discusses the principles of several of the major technologies available for performing homogeneous genetic analyses. Publications arising from the application of the technologies in a wide range of clinical areas are used to highlight and compare the potential advantages and shortcomings of the various technologies.
Content: This review is descriptive and focuses on three areas: the technologies available for performing homogeneous analysis, the clinical applications where the technologies are being used, and validation issues surrounding the acceptance of the technologies in the general clinical setting.
Summary: This review intends to give the reader a greater understanding of the various technologies available for performing homogeneous genetic testing in the clinical laboratory. Through insight into the principles and performance characteristics underlying these technologies, the end user can evaluate their value and limitations in the clinical diagnostic setting.
The following articles in journals at HighWire Press have cited this article:
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  J. M. Binstock Molecular Biology Techniques for Identifying Dermatophytes and Their Possible Use in Diagnosing Onychomycosis in Human Toenail: A Review J Am Podiatr Med Assoc, March 1, 2007; 97(2): 134 - 144. [Abstract] [Full Text] [PDF]
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 D. Rodriguez-Lazaro, D. A. Lewis, A. A. Ocampo-Sosa, U. Fogarty, L. Makrai, J. Navas, M. Scortti, M. Hernandez, and J. A. Vazquez-Boland Internally Controlled Real-Time PCR Method for Quantitative Species-Specific Detection and vapA Genotyping of Rhodococcus equi. Appl. Envir. Microbiol., June 1, 2006; 72(6): 4256 - 4263. [Abstract] [Full Text] [PDF]
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 C. Ronsin, A. Pillet, C. Bali, and G.-A. Denoyel Evaluation of the COBAS AmpliPrep-Total Nucleic Acid Isolation-COBAS TaqMan Hepatitis B Virus (HBV) Quantitative Test and Comparison to the VERSANT HBV DNA 3.0 Assay J. Clin. Microbiol., April 1, 2006; 44(4): 1390 - 1399. [Abstract] [Full Text] [PDF]
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C. Maynard, F. Berthiaume, K. Lemarchand, J. Harel, P. Payment, P. Bayardelle, L. Masson, and R. Brousseau Waterborne Pathogen Detection by Use of Oligonucleotide-Based Microarrays Appl. Envir. Microbiol., December 1, 2005; 71(12): 8548 - 8557. [Abstract] [Full Text] [PDF]
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 J. Cheng, Y. Zhang, and Q. Li Real-time PCR genotyping using displacing probes Nucleic Acids Res., April 15, 2004; 32(7): e61 - e61. [Abstract] [Full Text] [PDF]
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 C.-Y. Chen, S.-C. Shiesh, and S.-J. Wu Rapid Detection of K-ras Mutations in Bile by Peptide Nucleic Acid-mediated PCR Clamping and Melting Curve Analysis: Comparison with Restriction Fragment Length Polymorphism Analysis Clin. Chem., March 1, 2004; 50(3): 481 - 489. [Abstract] [Full Text] [PDF]
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M. B. Grace, C. B. McLeland, S. J. Gagliardi, J. M. Smith, W. E. Jackson III, and W. F. Blakely Development and Assessment of a Quantitative Reverse Transcription-PCR Assay for Simultaneous Measurement of Four Amplicons Clin. Chem., September 1, 2003; 49(9): 1467 - 1475. [Abstract] [Full Text] [PDF]
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P. S. Bernard and C. T. Wittwer Real-Time PCR Technology for Cancer Diagnostics Clin. Chem., August 1, 2002; 48(8): 1178 - 1185. [Abstract] [Full Text] [PDF]
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H. Millward, W. Samowitz, C. T. Wittwer, and P. S. Bernard Homogeneous Amplification and Mutation Scanning of the p53 Gene Using Fluorescent Melting Curves Clin. Chem., August 1, 2002; 48(8): 1321 - 1328. [Abstract] [Full Text] [PDF]
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Q. Li, G. Luan, Q. Guo, and J. Liang A new class of homogeneous nucleic acid probes based on specific displacement hybridization Nucleic Acids Res., January 15, 2002; 30(2): e5 - e5. [Abstract] [Full Text] [PDF]
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A. Ulvik and P. M. Ueland Single Nucleotide Polymorphism (SNP) Genotyping in Unprocessed Whole Blood and Serum by Real-Time PCR: Application to SNPs Affecting Homocysteine and Folate Metabolism Clin. Chem., November 1, 2001; 47(11): 2050 - 2053. [Full Text] [PDF]
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