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Clinical Chemistry 47: 1256-1262, 2001;
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(Clinical Chemistry. 2001;47:1256-1262.)
© 2001 American Association for Clinical Chemistry, Inc.

Articles

Quantitative Determination of Estradiol Fatty Acid Esters in Human Pregnancy Serum and Ovarian Follicular Fluid

Veera Vihma1, Herman Adlercreutz2, Aila Tiitinen3, Paula Kiuru4, Kristiina Wähälä4 and Matti J. Tikkanen1a

Departments of
1 Medicine, PB 340, and
3 Obstetrics and Gynecology, PB 140, Helsinki University Central Hospital, 00290 Helsinki, Finland.
2 Department of Clinical Chemistry, Folkhälsan Research Center, PB 60, University of Helsinki, FIN-00014 Helsinki, Finland.
4 Department of Organic Chemistry, PB 55, University of Helsinki, FIN-00014 Helsinki, Finland.


aAuthor for correspondence. Fax 358-9-47174013; e-mail matti.j.tikkanen{at}helsinki.fi.

Background: Lipophilic estradiol derivatives carried by lipoprotein particles in blood may mediate antioxidant or endocrine effects. We developed a new quantitative method to determine the concentration of circulating lipophilic estradiol fatty acid esters in human early- and late-pregnancy serum and in ovarian follicular fluid.

Methods: After extraction from serum or follicular fluid, estradiol fatty acid esters were separated from nonesterified estradiol by Sephadex LH-20 column chromatography. The estradiol ester fraction was hydrolyzed by saponification and further purified by several chromatographic steps. The hydrolyzed estradiol esters were measured by time-resolved fluoroimmunoassay.

Results: The average estradiol fatty acid ester concentration in serum increased 10-fold during pregnancy, from 40.4 pmol/L (expressed as pmol/L estradiol; range, 25.0–64.2 pmol/L) in early pregnancy (n = 8) to 404 pmol/L (196–731 pmol/L) in late pregnancy (n = 10). The ratio of estradiol ester to nonesterified estradiol remained relatively constant during pregnancy, at 0.4–0.6%. In 10 follicular fluid samples, the mean estradiol ester concentration was 106 nmol/L (56.9–262 nmol/L). Compared with serum, a greater proportion of estradiol in follicular fluid (3.0–10%) was in the esterified form.

Conclusion: The new method provides a means to measure circulating estradiol fatty acid ester concentrations in human pregnancy serum.




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Clin. Endocrinol. Metab.Home page
M. Badeau, V. Vihma, T. S. Mikkola, A. Tiitinen, and M. J. Tikkanen
Estradiol Fatty Acid Esters in Adipose Tissue and Serum of Pregnant and Pre- and Postmenopausal Women
J. Clin. Endocrinol. Metab., November 1, 2007; 92(11): 4327 - 4331.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
A. Hockerstedt, M. Jauhiainen, and M. J. Tikkanen
Lecithin/Cholesterol Acyltransferase Induces Estradiol Esterification in High-Density Lipoprotein, Increasing Its Antioxidant Potential
J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5088 - 5093.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
V. Vihma, A. Tiitinen, O. Ylikorkala, and M. J. Tikkanen
Quantitative Determination of Estradiol Fatty Acid Esters in Lipoprotein Fractions in Human Blood
J. Clin. Endocrinol. Metab., June 1, 2003; 88(6): 2552 - 2555.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
V. Vihma, S. Vehkavaara, H. Yki-Jarvinen, H. Hohtari, and M. J. Tikkanen
Differential Effects of Oral and Transdermal Estradiol Treatment on Circulating Estradiol Fatty Acid Ester Concentrations in Postmenopausal Women
J. Clin. Endocrinol. Metab., February 1, 2003; 88(2): 588 - 593.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. J Tikkanen, V. Vihma, M. Jauhiainen, A. Hockerstedt, H. Helisten, and M. Kaamanen
Lipoprotein-associated estrogens
Cardiovasc Res, November 1, 2002; 56(2): 184 - 188.
[Abstract] [Full Text] [PDF]


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