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Telomerase Detection in Body Fluids

¹ Program in Human Genetics, and
² Department of Pathology, University of Maryland at Baltimore, 737 W. Lombard St., Baltimore, MD 21201

^aAddress correspondence to this author at: Department of Pathology, University of Maryland at Baltimore, 7-22 MSTF, 10 South Pine St., Baltimore, MD 21201. Fax 410-706-8414; e-mail whighsmi{at}umaryland.edu.

Abstract

Background: Telomerase is a ribonucleoprotein that maintains chromosomal telomere length. Telomerase is not active in nonmalignant somatic cells, but is activated in most human cancers. Telomerase activity in easily obtainable body fluids that bathe tumors may be a useful cancer marker, especially when used in conjunction with conventional cytology.

Approach: Results from studies that assayed telomerase activity in easily obtainable body fluids are reviewed.

Content: The telomerase repeat amplification protocol (TRAP) assay has been used to measure telomerase activity in body fluids, including ascites, pleural effusions, pelvic washes, bronchial washings, bronchial lavage, urine, bladder washings, oral rinses, and plasma. Telomerase activity has sensitivities of 60–90% as a tumor marker with clinical specificities for cancer of 90%. Telomerase activity is more sensitive than conventional cytology, the sensitivity of which was 40–65% in various studies.

Summary: Telomerase activity in body fluids, as measured by the TRAP assay, is a sensitive potential tumor marker that might help increase the cancer detection rate and the cancer treatment success rate when combined with conventional cytology.

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