Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 1768-1771, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nexo, E.
Right arrow Articles by Nygard, O. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nexo, E.
Right arrow Articles by Nygard, O. K.
Related Collections
Right arrow General Clinical Chemistry
(Clinical Chemistry. 2002;48:1768-1771.)
© 2002 American Association for Clinical Chemistry, Inc.

Holo-Transcobalamin Is an Early Marker of Changes in Cobalamin Homeostasis. A Randomized Placebo-controlled Study

Ebba Nexo1a, Anne-Mette Hvas2, Øyvind Bleie3, Helga Refsum4, Sergey N. Fedosov5, Stein Emil Vollset6, Jorn Schneede6, Jan Erik Nordrehaug3, Per Magne Ueland6 and Ottar Kjell Nygard3,6

1 Department of Clinical Biochemistry, AKH, and
2 Department of Clinical Biochemistry, AAS, Aarhus University Hospital, DK 8000 Aarhus C, Denmark.

3 Institute of Medicine and
4 Department of Pharmacology, University of Bergen, N-5021 Bergen, Norway.

5 The Protein Chemistry Laboratory, Aarhus University, DK-5000 Aarhus C, Denmark.

6 Locus for Homocysteine and Related Vitamins, University of Bergen, N-5021 Bergen, Norway.

aAddress correspondence to this author at: Department of Clinical Biochemistry, AKH, Aarhus University Hospital, Norrebrogade 44, DK 8000 Aarhus C, Denmark. Fax 45-8949-3060; e-mail E.Nexo{at}dadlnet.dk.

Background: We examined the effect of oral vitamin B12 treatment on fluctuations in plasma total cobalamin and its binding proteins transcobalamin (TC) and haptocorrin (HC).

Methods: Patients (n = 88; age range, 38–80 years) undergoing coronary angiography (part of the homocysteine-lowering Western Norway B-Vitamin Intervention Trial) were allocated to daily oral treatment with (a) vitamin B12 (0.4 mg), folic acid (0.8 mg), and vitamin B6 (40 mg); (b) vitamin B12 and folic acid; (c) vitamin B6; or (d) placebo. EDTA blood was obtained before treatment and 3, 14, 28, and 84 days thereafter.

Results: The intraindividual variation for patients not treated with B12 was ~10% for plasma total cobalamin, total TC, apo-TC, and apo-HC, and <20% for holo-TC and TC saturation. In B12-treated patients, the maximum change in concentrations was observed already after 3 days for total TC (-16%), holo-TC (+54%), and TC saturation (+82%). At this time holo-HC (+20%) and plasma total cobalamin (+28%) showed an initial burst, but had increased further at 84 days. All changes were highly significant compared with the control group (P <0.0001).

Conclusions: Oral vitamin B12 treatment produces maximal effects on total TC, holo-TC, and TC saturation within 3 days, whereas maximal increases in holo-HC and plasma total cobalamin occur later. The results support the view that holo-TC is an early marker of changes in cobalamin homeostasis.




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Clin. Nutr.Home page
L. Hoey, J. Strain, and H. McNulty
Studies of biomarker responses to intervention with vitamin B-12: a systematic review of randomized controlled trials
Am. J. Clinical Nutrition, June 1, 2009; 89(6): 1981S - 1996S.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
A. Vogiatzoglou, H. Refsum, C. Johnston, S. M. Smith, K. M. Bradley, C. de Jager, M. M. Budge, and A. D. Smith
Vitamin B12 status and rate of brain volume loss in community-dwelling elderly
Neurology, September 9, 2008; 71(11): 826 - 832.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. G. Garrod, R. Green, L. H. Allen, D. M. Mungas, W. J. Jagust, M. N. Haan, and J. W. Miller
Fraction of Total Plasma Vitamin B12 Bound to Transcobalamin Correlates with Cognitive Function in Elderly Latinos with Depressive Symptoms
Clin. Chem., July 1, 2008; 54(7): 1210 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Brady, L. Wilson, L. McGregor, E. Valente, and L. Orning
Active B12: A Rapid, Automated Assay for Holotranscobalamin on the Abbott AxSYM Analyzer
Clin. Chem., March 1, 2008; 54(3): 567 - 573.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
M. M. Murphy, A. M. Molloy, P. M. Ueland, J. D. Fernandez-Ballart, J. Schneede, V. Arija, and J. M. Scott
Longitudinal Study of the Effect of Pregnancy on Maternal and Fetal Cobalamin Status in Healthy Women and Their Offspring
J. Nutr., August 1, 2007; 137(8): 1863 - 1867.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
K. M von Castel-Roberts, A. L. Morkbak, E. Nexo, C. A Edgemon, D. R Maneval, J. J Shuster, J. F Valentine, G. P. Kauwell, and L. B Bailey
Holo-transcobalamin is an indicator of vitamin B-12 absorption in healthy adults with adequate vitamin B-12 status
Am. J. Clinical Nutrition, April 1, 2007; 85(4): 1057 - 1061.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
C. McCracken, P. Hudson, R. Ellis, A. McCaddon, and the Medical Research Council Cognitive Function an
Methylmalonic acid and cognitive function in the Medical Research Council Cognitive Function and Ageing Study
Am. J. Clinical Nutrition, December 1, 2006; 84(6): 1406 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. L. Morkbak, A.-M. Hvas, Z. Lloyd-Wright, T. A.B. Sanders, O. Bleie, H. Refsum, O. K. Nygaard, and E. Nexo
Effect of Vitamin B12 Treatment on Haptocorrin
Clin. Chem., June 1, 2006; 52(6): 1104 - 1111.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Obeid, A. L. Morkbak, W. Munz, E. Nexo, and W. Herrmann
The Cobalamin-Binding Proteins Transcobalamin and Haptocorrin in Maternal and Cord Blood Sera at Birth
Clin. Chem., February 1, 2006; 52(2): 263 - 269.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. W. Miller, M. G. Garrod, A. L. Rockwood, M. M. Kushnir, L. H. Allen, M. N. Haan, and R. Green
Measurement of Total Vitamin B12 and Holotranscobalamin, Singly and in Combination, in Screening for Metabolic Vitamin B12 Deficiency
Clin. Chem., February 1, 2006; 52(2): 278 - 285.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
H. Refsum, C. Johnston, A. B. Guttormsen, and E. Nexo
Holotranscobalamin and Total Transcobalamin in Human Plasma: Determination, Determinants, and Reference Values in Healthy Adults
Clin. Chem., January 1, 2006; 52(1): 129 - 137.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
R. A. Dhonukshe-Rutten, M. van Zutphen, L. C. de Groot, S. J. Eussen, H. J Blom, and W. A van Staveren
Effect of supplementation with cobalamin carried either by a milk product or a capsule in mildly cobalamin-deficient elderly Dutch persons
Am. J. Clinical Nutrition, September 1, 2005; 82(3): 568 - 574.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
K. M von Castel-Dunwoody, G. P. Kauwell, K. P Shelnutt, J. D Vaughn, E. R Griffin, D. R Maneval, D. W Theriaque, and L. B Bailey
Transcobalamin 776C->G polymorphism negatively affects vitamin B-12 metabolism
Am. J. Clinical Nutrition, June 1, 2005; 81(6): 1436 - 1441.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
O. Bleie, H. Refsum, P. M. Ueland, S. E. Vollset, A. B. Guttormsen, E. Nexo, J. Schneede, J. E. Nordrehaug, and O. Nygard
Changes in basal and postmethionine load concentrations of total homocysteine and cystathionine after B vitamin intervention
Am. J. Clinical Nutrition, September 1, 2004; 80(3): 641 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. V. Bor, E. Nexo, and A.-M. Hvas
Holo-Transcobalamin Concentration and Transcobalamin Saturation Reflect Recent Vitamin B12 Absorption Better than Does Serum Vitamin B12
Clin. Chem., June 1, 2004; 50(6): 1043 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
P. I. Holm, O. Bleie, P. M. Ueland, E. A. Lien, H. Refsum, J. E. Nordrehaug, and O. Nygard
Betaine as a Determinant of Postmethionine Load Total Plasma Homocysteine Before and After B-Vitamin Supplementation
Arterioscler Thromb Vasc Biol, February 1, 2004; 24(2): 301 - 307.
[Abstract] [Full Text]

Home page
 Clin. Chem.Home page
H. Refsum, A. D. Smith, P. M. Ueland, E. Nexo, R. Clarke, J. McPartlin, C. Johnston, F. Engbaek, J. Schneede, C. McPartlin, et al.
Facts and Recommendations about Total Homocysteine Determinations: An Expert Opinion
Clin. Chem., January 1, 2004; 50(1): 3 - 32.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. McCaddon, P. Hudson, C. McCracken, R. Ellis, and A. McCaddon
Biological Variation of Holo-Transcobalamin in Elderly Individuals
Clin. Chem., September 1, 2003; 49(9): 1542 - 1544.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
H. Zetterberg, E. Nexo, B. Regland, L. Minthon, R. Boson, M. Palmer, L. Rymo, and K. Blennow
The Transcobalamin (TC) Codon 259 Genetic Polymorphism Influences Holo-TC Concentration in Cerebrospinal Fluid from Patients with Alzheimer Disease
Clin. Chem., July 1, 2003; 49(7): 1195 - 1198.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. V. Bor, H. Refsum, M. R. Bisp, O. Bleie, J. Schneede, J. E. Nordrehaug, P. M. Ueland, O. K. Nygard, and E. Nexo
Plasma Vitamin B6 Vitamers before and after Oral Vitamin B6 Treatment: A Randomized Placebo-controlled Study
Clin. Chem., January 1, 2003; 49(1): 155 - 161.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.