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Clinical Chemistry 48: 291-300, 2002;
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(Clinical Chemistry. 2002;48:291-300.)
© 2002 American Association for Clinical Chemistry, Inc.

Apolipoprotein E in Apolipoprotein B (apo B)- and Non-apo B-containing Lipoproteins in 3523 Participants in the Stanislas Cohort: Biological Variation and Genotype-specific Reference Limits

Françoise Schiele1, Monique Vincent-Viry1, Marjorie Starck1, Brigitte Beaud1, Geneviève Hennache2, Gérard Siest1, Sophie Visvikis1 and Bernard Herbeth1a

1 Centre de Médecine Préventive, INSERM U525, 2 Rue du Doyen Jacques Parisot, F54500 Vandoeuvre-lès-Nancy, France.

2 Sebia, 23 Rue Maximilien Robespierre, F92130 Issy-les-Moulineaux, France.

aAuthor for correspondence. Fax 33-3-83-44-87-21; e-mail Bernard.Herbeth{at}cmp.u-nancy.fr.

Background: Apolipoprotein (apo) E is a component of two major classes of plasma lipoproteins, apo B- (apo E-LpB) and non-apo B-containing (apo E-Lp-non-B) lipoproteins. The factors that affect total apo E in particles [lipoprotein E (LpE), apo E-Lp-non-B, and apo E-LpB], are incompletely characterized.

Methods: We studied the determinants of these lipoparticles in a sample population of presumably healthy individuals: 1784 children (age range, 8–18 years) and 1739 adults (age range, 19–50 years). Serum concentrations of LpE and apo E-Lp-non-B were measured by electroimmunoassays, and the concentration of apo E-LpB was calculated by a difference method.

Results: Serum LpE and apo E-Lp-non-B were higher in females than in males. Their concentrations decreased with age until 20–25 years and then increased in men but not in women. apo E-LpB concentrations increased up to 20–25 years and were similar in both sexes. Thereafter, adult men had higher values than women. Individuals carrying the {epsilon}2 allele had higher mean apo E-Lp-non-B concentrations and lower apo E-LpB concentrations than did individuals carrying the {epsilon}3 allele. Individuals with the {epsilon}4 allele showed an inverse profile compared with those with the {epsilon}2 allele. Age, gender, the common apo E polymorphism, puberty, serum lipid concentrations, and alcohol consumption were significantly associated with total LpE, apo E-Lp-non-B, and apo E-LpB concentrations. Reference limits were established according to age, gender, and the common apo E polymorphism.

Conclusions: Because measurements of LpE, apo E-Lp-non-B, and apo E-LpB concentrations may improve cardiovascular risk assessment, the proposed reference limits will aid interpretation of the results in clinical or therapeutic trials.






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