Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 859-868, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fredericks, S.
Right arrow Articles by Holt, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fredericks, S.
Right arrow Articles by Holt, D. W.
Related Collections
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2002;48:859-868.)
© 2002 American Association for Clinical Chemistry, Inc.

Cardiac Troponin T and Creatine Kinase MB Content in Skeletal Muscle of the Uremic Rat

Salim Fredericks1a, Joanne F. Murray2, Nicholas D. Carter3, Alistair M.S. Chesser4, Stavroula Papachristou1, Magdi M. Yaqoob4, Paul O. Collinson5, David Gaze5 and David W. Holt1

1 Analytical Unit, Cardiological Sciences,
2 Obstetrics & Gynaecology and Physiology,
3 Medical Genetics Unit, and
5 Department of Chemical Pathology, St. George’s Hospital Medical School, London SW17 0RE, United Kingdom.

4 Department of Renal Medicine, Queen Mary and Westfield College (QMWC) School of Medicine, St. Bartholomew’s Hospital Medical College, London EC1M 6BQ, United Kingdom.

aAddress correspondence to this author at: Analytical Unit, Cardiological Sciences, St. George’s Hospital Medical School, London SW17 0RE, United Kingdom. Fax: 44-0-20-8767-9687; e-mail frederic{at}sghms.ac.uk.

Background: The assertion that creatine kinase MB (CK-MB) and the developmental isoforms of cardiac troponin T (cTnT) are expressed by skeletal muscle in some clinical settings is an extrapolation from nonuremic rodent studies. We studied the content of CK-MB and cTnT in skeletal muscle of the renal-insufficient rat.

Methods: Skeletal muscles (gastrocnemius) were collected from both five-sixths nephrectomized rats (n = 11) and sham-operated controls (n = 11). cTnT content was analyzed by Elecsys (Roche), immunoblotting, and immunohistochemistry with antibodies M7 and M11-7 (Roche). CK isoenzymes were analyzed electrophoretically.

Results: Trace concentrations of cTnT were detected in some of the skeletal muscle samples [controls (3 of 11) and uremic rats (1 of 11)] at concentrations <0.01% of that detected in heart. By contrast, positive staining appeared in both groups with M11-7 by immunoblotting and immunohistochemistry. No immunoreactivity was detected in skeletal muscle using M7 in the immunoblot format, although immunoreactivity was detected by immunohistochemistry in all samples. The median percentages of CK-MB were 6.0% and 4.1% for the skeletal muscle from control and uremic rats, respectively.

Conclusion: The detection of cTnT and CK-MB in skeletal muscle does not differ for uremic rats compared with sham-operated controls. cTnT isoforms detected by qualitative methods are not detected with the cTnT immunoassay. Observations with rodents should not necessarily be extrapolated to humans.




The following articles in journals at HighWire Press have cited this article:


Home page
 Toxicol PatholHome page
D. B. Walker
Serum Chemical Biomarkers of Cardiac Injury for Nonclinical Safety Testing
Toxicol Pathol, January 1, 2006; 34(1): 94 - 104.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.