Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 1084-1093, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Figures
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jonsson, M.
Right arrow Articles by Carlson, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jonsson, M.
Right arrow Articles by Carlson, J.
Related Collections
Right arrow Laboratory Management
Right arrow Clinical Immunology
Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2002;48:1084-1093.)
© 2002 American Association for Clinical Chemistry, Inc.

Computer-supported Interpretation of Protein Profiles after Capillary Electrophoresis

Magnus Jonsson1 and Joyce Carlsona1

1 Department of Clinical Chemistry, University Hospital MAS, S-205 02 Malmö, Sweden.

aAuthor for correspondence. Fax 46-40-336286; e-mail Joyce.Carlson{at}klkemi.mas.lu.se.

Background: Electrophoretic patterns of proteins in serum/plasma are useful in the diagnosis and evaluation of many diseases. Capillary zone electrophoresis (CZE) allows rapid and automated protein separation and produces digital absorbance data, appropriate for mathematical analysis. We previously demonstrated success in detection of monoclonal immunoglobulins in such a system. This study tests new algorithms to produce rapid standardized computer-supported interpretation of the entire electropherogram.

Methods: Data from Beckman Paragon CZE 2000 electropherograms were compared with quantitative protein data from >800 routine clinical samples. Algorithms were designed to produce semiquantitative analyses of major proteins and to define different patterns of inflammation based on the electropherogram.

Results: The algorithms produced reliable semiquantitative evaluations of prealbumin, albumin, {alpha}1-antitrypsin, haptoglobin, and transferrin, but were less accurate for {alpha}1-acid glycoprotein. Some genetic variants of albumin and deficiency variants of {alpha}1-antitrypsin were easily recognized. Complex clinical traits such as degree and type of inflammation could be evaluated. When used together with previously developed algorithms addressing immunoglobulins, the new algorithms provide relevant clinical interpretation. Selected outputs indicate the need for reflex testing or evaluation by specialists.

Conclusions: Automation of both electrophoresis and interpretation can provide a rapid, inexpensive, standardized analysis that can hopefully improve the diagnostic information and clinical outcome for large groups of patients. It also provides objective criteria for clinical interpretations, to be validated or adjusted in future clinical studies.




The following articles in journals at HighWire Press have cited this article:


Home page
 ChestHome page
E. Piitulainen, J. Carlson, K. Ohlsson, and T. Sveger
{alpha}1-Antitrypsin Deficiency in 26-Year-Old Subjects: Lung, Liver, and Protease/Protease Inhibitor Studies
Chest, October 1, 2005; 128(4): 2076 - 2081.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. Gay-Bellile, D. Bengoufa, P. Houze, D. Le Carrer, M. Benlakehal, B. Bousquet, B. Gourmel, and T. Le Bricon
Automated Multicapillary Electrophoresis for Analysis of Human Serum Proteins
Clin. Chem., November 1, 2003; 49(11): 1909 - 1915.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Regeniter, J. U. Steiger, A. Scholer, P. R. Huber, and W. H. Siede
Windows to the Ward: Graphically Oriented Report Forms. Presentation of Complex, Interrelated Laboratory Data for Electrophoresis/Immunofixation, Cerebrospinal Fluid, and Urinary Protein Profiles
Clin. Chem., January 1, 2003; 49(1): 41 - 50.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.