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Clinical Chemistry 48: 1225-1231, 2002;
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(Clinical Chemistry. 2002;48:1225-1231.)
© 2002 American Association for Clinical Chemistry, Inc.

Application of a Multigene Reverse Transcription-PCR Assay for Detection of Mammaglobin and Complementary Transcribed Genes in Breast Cancer Lymph Nodes

Barbara K. Zehentner1, Davin C. Dillon1, Yuqiu Jiang1, Jiangchun Xu1, Angela Bennington1, David A. Molesh1, XinQun Zhang1, Steven G. Reed1, David Persing1 and Raymond L. Houghtona1

1 Corixa, 1124 Columbia St., Seattle, WA 98104.

aAuthor for correspondence. Fax 206-754-5917; e-mail Houghton{at}corixa.com.

Background: Mammaglobin mRNA expression is found in 70–80% of primary and metastatic breast tumor biopsies. The potential breast tumor markers B305D, B726P, and {gamma}-aminobutyrate type A receptor {pi} subunit (GABA{pi}) complement the expression of mammaglobin. Collectively the expression profile of these four genes could be used as a diagnostic and prognostic indicator for breast cancer.

Methods: A multigene reverse transcription-PCR (RT-PCR) assay was established to detect the expression of mammaglobin, GABA{pi}, B305D, and B726P simultaneously. Specific primers and TaqMan® probes were used to analyze combined mRNA expression profiles in primary breast tumors and metastatic lymph node specimens.

Results: The multigene RT-PCR assay detected substantial expression signals in 27 of 27 primary tumor and 50 of 50 metastatic breast lymph node samples. Specificity studies demonstrated no significant expression signal in 27 non-breast cancer lymph nodes, in 22 various healthy tissue samples, or in 14 colon tumor samples.

Conclusion: The novel RT-PCR-based assay described here provides a sensitive detection system for disseminated breast tumor cells in lymph nodes. In addition, this multigene assay could also be used to test peripheral blood and bone marrow samples.




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