| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Saarland, D-66421 Homburg (Saar), Germany.
aAuthor for correspondence. Fax 49-6841-16-26051; e-mail hans.maurer{at}uniklinik-saarland.de.
Background: Enantioselective analysis of amphetamine (AM) or methamphetamine (MA) in urine is already a well-established tool for differentiation of illicit from therapeutic ingestion of AM or MA derivatives. However, because of the increasing importance of plasma or serum in analytical toxicology, a method for enantioselective analysis of AM and MA in these matrices is needed.
Methods: AM and/or MA were extracted from 0.2 mL of blood plasma or serum by mixed-mode solid-phase extraction. After derivatization with S-(-)-heptafluorobutyrylprolyl chloride, the resulting diastereomers were separated by gas chromatography on a HP-5MS column during a 15-min program and detected by mass spectrometry in the negative-ion chemical ionization mode (NICI-GC-MS). The method was fully validated and applied to >50 samples from authentic toxicology cases.
Results: The derivatized AM and MA enantiomers were well separated and sensitively detected. The method was linear from 5 to 250 µg/L per enantiomer with analytical recoveries, accuracy, and within- and between-run precision well within required limits. Extraction yields were 88.9–98.6%. Implications of concentrations and enantiomeric composition of AM and MA in the authentic samples were considered.
Conclusions: This sensitive, reliable, rapid NICI-GC-MS assay is suitable for enantioselective determination of AM and MA in blood plasma or serum samples.
The following articles in journals at HighWire Press have cited this article:
|
|
 |
|
  A. E. Schwaninger, M. R. Meyer, J. Zapp, and H. H. Maurer The Role of Human UDP-Glucuronyltransferases on the Formation of the Methylenedioxymethamphetamine (Ecstasy) Phase II Metabolites R- and S-3-Methoxymethamphetamine 4-O-Glucuronides Drug Metab. Dispos., November 1, 2009; 37(11): 2212 - 2220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Meyer, F. T. Peters, and H. H. Maurer The Role of Human Hepatic Cytochrome P450 Isozymes in the Metabolism of Racemic 3,4-Methylenedioxy-Methamphetamine and Its Enantiomers Drug Metab. Dispos., November 1, 2008; 36(11): 2345 - 2354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. T. Peters, N. Samyn, T. Kraemer, W. J. Riedel, and H. H. Maurer Negative-Ion Chemical Ionization Gas Chromatography-Mass Spectrometry Assay for Enantioselective Measurement of Amphetamines in Oral Fluid: Application to a Controlled Study with MDMA and Driving Under the Influence Cases Clin. Chem., April 1, 2007; 53(4): 702 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. T. Peters, N. Samyn, C. T.J. Lamers, W. J. Riedel, T. Kraemer, G. de Boeck, and H. H. Maurer Drug Testing in Blood: Validated Negative-Ion Chemical Ionization Gas Chromatographic-Mass Spectrometric Assay for Enantioselective Measurement of the Designer Drugs MDEA, MDMA, and MDA and Its Application to Samples from a Controlled Study with MDMA Clin. Chem., October 1, 2005; 51(10): 1811 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-G. Shyu, B.-W. Wang, Y.-H. Yang, S.-C. Tsai, S. Lin, and C.-C. Lee Amphetamine activates connexin43 gene expression in cultured neonatal rat cardiomyocytes through JNK and AP-1 pathway Cardiovasc Res, July 1, 2004; 63(1): 98 - 108. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
