Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 48: 1526-1538, 2002;
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cobbaert, C.
Right arrow Articles by Jansen, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cobbaert, C.
Right arrow Articles by Jansen, R.
Related Collections
Right arrow Laboratory Management
Right arrow Lipids, Lipoproteins, and Cardiovascular Risk Factors
(Clinical Chemistry. 2002;48:1526-1538.)
© 2002 American Association for Clinical Chemistry, Inc.

Selection, Preparation, and Characterization of Commutable Frozen Human Serum Pools as Potential Secondary Reference Materials for Lipid and Apolipoprotein Measurements: Study within the Framework of the Dutch Project "Calibration 2000"

Christa Cobbaert1,2a, Cas Weykamp3, Henk Baadenhuijsen4, Aldy Kuypers4, Jan Lindemans2 and Rob Jansen5

1 Amphia Hospital, 4819 EV Breda, The Netherlands.

2 Lipid Reference Laboratory, University Hospital Rotterdam, 3000 CA Rotterdam, The Netherlands.

3 Queen Beatrix Hospital, 7100 GG Winterswijk, The Netherlands.

4 Dutch Foundation for Quality Assessment in Clinical Laboratories (SKZL), University Hospital Nijmegen, 6500 HB Nijmegen, The Netherlands.

5 St. Anna Hospital, 5660 AB Geldrop, The Netherlands.

aAddress correspondence to this author at: Amphia Hospital, Langendijk, Department of Clinical Chemistry, Langendijk 75, 4819 EV Breda, The Netherlands. Fax 31-76-5277043; e-mail cobbaert{at}worldonline.nl.

Background: The Dutch project "Calibration 2000" aims at harmonization of laboratory results via calibration by development of matrix-based secondary reference materials. We considered the selection, preparation, and characterization of 34 potential reference materials (PRMs).

Methods: Sixteen PRMs were prepared either strictly according to the NCCLS C37-A protocol or in a less stringent and more convenient way. In addition, 18 commercial, so-called human serum-based calibrators or controls were purchased and tested. Lipoprotein integrity was evaluated by examining the physicochemical characteristics of the materials. Commutability of the PRMs was assessed in 86 Dutch clinical laboratories, using a multicenter split-patient-sample between-field-method (twin-study) design. Normalized residuals of the PRMs with respect to the patient regression lines were calculated; in addition, the extra contribution of each PRM to the total measurement uncertainty (CVNetto) was calculated. On the basis of these results, the most native PRM was selected to investigate its potential to reduce interlaboratory variation and to improve lipid and apolipoprotein standardization.

Results: In general, only the NCCLS C37-A-type materials displayed normalized residuals below the decision limit for commutability and had small CVNetto values ranging between 0 and 3.8%. This contrasts with the findings in regularly pooled frozen sera and lyophilized cryoprotected PRMs. In two subsequent external quality assessment surveys, the NCCLS type C37-A materials contributed to reducing the intermethod lipid and (apo)lipoprotein variation to ~2–4%.

Conclusions: NCCLS C37-A materials have a strong potential as secondary reference materials, not only for cholesterol but also for HDL-cholesterol, LDL-cholesterol, triglyceride, and apolipoprotein measurements.




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
H. Baadenhuijsen, H. Steigstra, C. Cobbaert, A. Kuypers, C. Weykamp, and R. Jansen
Commutability Assessment of Potential Reference Materials Using a Multicenter Split-Patient-Sample Between-Field-Methods (Twin-Study) Design: Study within the Framework of the Dutch Project "Calibration 2000"
Clin. Chem., September 1, 2002; 48(9): 1520 - 1525.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the American Association for Clinical Chemistry.