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Clinical Chemistry 49: 594-600, 2003; 10.1373/49.4.594
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Right arrow Proteomics and Protein Markers
(Clinical Chemistry. 2003;49:594-600.)
© 2003 American Association for Clinical Chemistry, Inc.

New ELISA System for Myeloid-related Protein Complex (MRP8/14) and Its Clinical Significance as a Sensitive Marker for Inflammatory Responses Associated with Transplant Rejection

Masaki Ikemoto1,a, Tomoko Tanaka1, Yuri Takai1, Hiroshi Murayama2, Koichi Tanaka3 and Masatoshi Fujita1

1 College of Medical Technology, Kyoto University, Kyoto 606-8507, Japan.

2 Immunological Laboratory, Diagnostic Division, Yamasa Shoyu Co., Ltd, Choshi, Chiba 288-0056, Japan.

3 Department of Transplantation Medicine, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.

aAddress correspondence to this author at: College of Medical Technology, Kyoto University, 53 Kawaharacho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. Fax 81-75-751-3909; e-mail mmas{at}kuhp.kyoto-u.ac.jp.

Background: C-reactive protein (CRP), a useful marker for inflammatory diseases, is not always sensitive to inflammatory reaction in the liver or other tissues. The aim of this study was to develop a sensitive and specific method for detecting inflammatory responses associated with transplant rejection.

Methods: We developed a new, highly sensitive ELISA system for the measurement of serum human myeloid-related protein complex (MRP8/14), using monoclonal antibodies against MRP8/14, and applied it to specimens obtained from patients undergoing small intestine or liver transplantation.

Results: This assay could detect MRP8/14 concentrations as low as 2 µg/L. Within-run CVs were 3.7–6.1% and between-day CVs were 5.6–8.7% for MRP8/14 concentrations of 117-3300 µg/L. Mean recovery was 104% (range, 80–128%). We observed a marked increase in serum MRP8/14 postoperatively in most recipients of transplants, followed by an increase in CRP 1–7 days after the increase in the complex. The increase in serum MRP8/14 occurred simultaneously with permeation of lymphocytes into the transplanted tissues as a result of rejection of the graft tissues.

Conclusions: Accurate measurement of serum MRP8/14 provides a useful clinical diagnostic method tool for detecting inflammation associated with rejection of transplanted tissues.




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S Miyamoto, M Ueda, M Ikemoto, T Naruko, A Itoh, S Tamaki, R Nohara, F Terasaki, S Sasayama, and M Fujita
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[Abstract] [Full Text] [PDF]


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