Clinical Chemistry
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Clinical Chemistry 49: 868-872, 2003; 10.1373/49.6.868
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(Clinical Chemistry. 2003;49:868-872.)
© 2003 American Association for Clinical Chemistry, Inc.

Convenient and Effective Method for Removing Fibrinogen from Serum Specimens before Protein Electrophoresis

Ling L. Qiu1, Stanley S. Levinson1,2, Kristen L. Keeling1 and Ronald J. Elin1,a

1 Department of Pathology and Laboratory Medicine, University of Louisville School of Medicine, Louisville, KY 40202.

2 Laboratory Service, Veterans’ Administration Medical Center, Louisville, KY 40206-1466.

aAuthor for correspondence. Fax 502-852-8299; e-mail rjelin01{at}gwise.louisville.edu.

Background: Fibrinogen in serum specimens can be misinterpreted on protein electrophoresis as a monoclonal protein. We evaluated selective precipitation of fibrinogen with ethanol.

Methods: Pooled human plasma was mixed with absolute ethanol or saline (final concentrations of 40, 80, 100, 120, and 160 mL/L) and incubated at 4 °C overnight or placed in an ice bath for 15 min. After centrifugation, the supernatants and resuspended pellets were used for protein electrophoresis and quantitative measurements of protein and fibrinogen.

Results: The fibrinogen band was effectively eliminated from the electrophoretic pattern in the plasma samples treated with ethanol at 100 mL/L and incubated in an ice bath for 15 min without a significant change in immunoglobulin concentrations. The 100 mL/L ethanol did not noticeably change the electrophoretic pattern of monoclonal immunoglobulins. This approach allowed analysis of a sample collected from an arteriovenous shunt kept open with heparin.

Conclusions: Ethanol, 100 mL/L, can selectively precipitate fibrinogen without significantly interfering with the immunoglobulins. The precipitation process can be completed in 15 min at 0–4 °C and can avoid the need to obtain another blood sample.




The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
K. Gijbels, G. Marien, and X. Bossuyt
Ethanol Precipitation Is Not Reliable for Selectively Removing Nonmonoclonal Peaks Seen in the Fibrinogen Region on Capillary Zone Electrophoresis of Serum Proteins
Clin. Chem., October 1, 2004; 50(10): 1880 - 1881.
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 Clin. Chem.Home page
Y. Ibrahim, M. Volkmann, R. Hassoun, W. Fiehn, H. Rossmann, S. S. Levinson, and R. J. Elin
Serum Protein Electrophoresis: Reptilase Treatment Is Superior to Ethanol Precipitation for Specific Removal of Fibrinogen from Heparinized Plasma Samples * Drs. Levinson and Elin respond:
Clin. Chem., June 1, 2004; 50(6): 1100 - 1101.
[Full Text] [PDF]


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