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Clinical Chemistry 49: 1074-1080, 2003; 10.1373/49.7.1074
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(Clinical Chemistry. 2003;49:1074-1080.)
© 2003 American Association for Clinical Chemistry, Inc.

Molecular Beacon Reverse Transcription-PCR of Human Chorionic Gonadotropin-ß-3, -5, and -8 mRNAs Has Prognostic Value in Breast Cancer

Paul N. Span1,a, Peggy Manders1,2, Joop J.T.M. Heuvel1, Chris M.G. Thomas1, Remko R. Bosch1,3, Louk V.A.M. Beex2 and C.G.J. (Fred) Sweep1

Departments of
1 Chemical Endocrinology,
2 Medical Oncology, and
3 Endocrinology, University Medical Center Nijmegen, 6500 HB Nijmegen, The Netherlands.

aAddress correspondence to this author at: 530 Department of Chemical Endocrinology, University Medical Center Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands. Fax 31-24-3541484; e-mail p.span{at}ace.umcn.nl.

Background: The ß-subunit of human chorionic gonadotropin (hCG) is encoded by four genes, of which expression of the hCGß-3, -5, and -8 genes could have prognostic value in breast cancer.

Methods: Applying a new, modified Molecular Beacon reverse transcription-PCR assay, we investigated the prognostic value of the hCGß-3, -5, and -8 gene transcripts in 129 sporadic unilateral breast cancer samples from patients with a median follow-up of 62.3 months.

Results: Expression of hCGß-3, -5, -8 was significantly (P = 0.020) associated with relapse-free survival (RFS). In multivariate survival analysis, hCGß-3, -5, and -8 maintained prognostic value for RFS, with high expression predicting shorter RFS (P = 0.015; hazard ratio, 2.25; 95% confidence interval, 1.17–4.34). Only 1 of 24 (4%) node-negative patients with low hCGß-3, -5, -8 expression relapsed, in contrast to 7 of 26 (27%) patients with high expression (P = 0.046).

Conclusions: Expression of hCGß-3, -5, -8, which differ by only one nucleotide from other hCGß genes, can be assessed by our modified Molecular Beacon assay in breast cancer tissues. Expression of hCGß-3, -5, -8 has independent, prognostic value for RFS in breast cancer and may help identify node-negative patients with poor prognosis.




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