Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 49: 1125-1132, 2003; 10.1373/49.7.1125
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, K.-Z.
Right arrow Articles by Mantsch, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, K.-Z.
Right arrow Articles by Mantsch, H. H.
Related Collections
Right arrow Molecular Diagnostics and Genetics
Right arrow Proteomics and Protein Markers
Right arrow Hematology
(Clinical Chemistry. 2003;49:1125-1132.)
© 2003 American Association for Clinical Chemistry, Inc.

Infrared Spectroscopic Identification of ß-Thalassemia

Kan-Zhi Liu1,a, Kam Sze Tsang2, Chi Kong Li3, R. Anthony Shaw1 and Henry H. Mantsch1

1 Institute for Biodiagnostics, National Research Council of Canada, 435 Ellice Ave., Winnipeg, Manitoba, R3B 1Y6 Canada.
Departments of
2 Anatomical & Cellular Pathology and
3 Pediatrics, The Chinese University of Hong Kong, Cancer Center, Prince of Wales Hospital, Hong Kong, Peoples Republic of China.

aAuthor for correspondence. Fax 204-984-4572; e-mail Kan-Zhi.Liu{at}nrc.ca.

Background: The aim of this study was to investigate the potential of infrared (IR) spectroscopy as a fast and reagent-free adjunct tool in the diagnosis and screening of ß-thalassemia.

Methods: Blood was obtained from 56 patients with ß-thalassemia major, 1 patient with hemoglobin H disease, and 35 age-matched controls. Hemolysates of blood samples were centrifuged to remove stroma. IR absorption spectra were recorded for duplicate films dried from 5 µL of hemolysate. Differentiation between the two groups of hemoglobin spectra was by two statistical methods: an unsupervised cluster analysis and a supervised linear discriminant analysis (LDA).

Results: The IR spectra revealed changes in the secondary structure of hemoglobin from ß-thalassemia patients compared with that from controls, in particular, a decreased {alpha}-helix content, an increased content of parallel and antiparallel ß-sheets, and changes in the tyrosine ring absorption band. The hemoglobin from ß-thalassemia patients also showed an increase in the intensity of the IR bands from the cysteine -SH groups. The unsupervised cluster analysis, statistically separating spectra into different groups according to subtle IR spectral differences, allowed separation of control hemoglobin from ß-thalassemia hemoglobin spectra, based mainly on differences in protein secondary structure. The supervised LDA method provided 100% classification accuracy for the training set and 98% accuracy for the validation set in partitioning control and ß-thalassemia samples.

Conclusion: IR spectroscopy holds promise in the clinical diagnosis and screening of ß-thalassemia.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Association for Clinical Chemistry.