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Clinical Chemistry 5: 36-44, 1959;
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Clinical Chemistry, Vol 5, 36-44, Copyright © 1959 by the American Association for Clinical Chemistry

The Rapid Determination of Calcium in Biologic Material

Harold D. Appleton 1, Marcia West 1, Mary Mandel 1, and Angelo M. Sala 1

1 Department of Clinical Chemistry, Division of Pathology, Metropolitan Hospital, New York, N. Y.

A rapid procedure for the routine determination of calcium in serum and urine has been described. 0.1 ml. of serum is titrated directly with standard EDTA, using either a calcein-KCl mixture or a calcein-thymolphthalein-KCl mixture. The end point with either indicating mixture is sharp. Excellent agreement is shown with the oxalate isolation and permanganate titration procedure.

Submitted on September 27, 1958






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Copyright © 1959 by the American Association for Clinical Chemistry.