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Clinical Chemistry 5: 119-126, 1959;
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Clinical Chemistry, Vol 5, 119-126, Copyright © 1959 by the American Association for Clinical Chemistry

Adaptation of an Alkaline Phosphatase Method for Automatic Colorimetric Analysis

Walton H. Marsh 1, Benjamin Fingerhut 1, and Elaine Kirsch 1

1 Department of Pathology, State University of New York, College of Medicine, Brooklyn, N. Y., and Department of Clinical Chemistry, Division of Laboratories, Kings County Hospital, Brooklyn, N. Y.

The alkaline phosphatase method of Kind and King was adapted to an automated recording colorimeter. The precision of the automated method (1 standard deviation as per cent of the mean value) was ±1.7 and for the manual method ±3.6 per cent. The color produced was proportional to the enzyme concentration by both methods, and recoveries of added phenol were satisfactory. In more than 150 serum specimens surveyed for enzyme activity, over 95 per cent of the results (2 standard deviations) of the 2 methods in the range 3.4-129 agree to within ±2.8 King-Armstrong units/1OO ml.

Submitted on November 21, 1958




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Copyright © 1959 by the American Association for Clinical Chemistry.