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Clinical Chemistry 5: 149-160, 1959;
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Clinical Chemistry, Vol 5, 149-160, Copyright © 1959 by the American Association for Clinical Chemistry

Staining Properties of Oil Red O and a Method of Partial Purification of the Commercial Product

Henn Kutt 1 and Theodore T. Tsaltas 1

1 Neurological Service of the Second (Cornell) Medical Division, Bellevue Hospital; the Department of Medicine, Cornell University Medical College; and the Department of Pathology, New York University College of Medicine, New York, N. Y.

1. A yellow-brown component of commercial oil red O separated by our technic of paper chromatography is shown to stain human serum proteins, particularly those that are coagulated by heat. The patterns of serum electropherograms obtained by coloring with the crude oil red O solution consist of a red lipid pattern superimposed on a brownish protein pattern.

2. A method is described for partial removal of the nonred components from the crude oil red O. Satisfactory coloration of lipoproteins was obtained with the "purified" oil red O.

3. It is demonstrated that areas of paper strip covered with protein may stain lighter with some oil red O fractions than the background filter paper.

Submitted on November 22, 1958






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Copyright © 1959 by the American Association for Clinical Chemistry.