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Clinical Chemistry 5: 284-296, 1959;
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Clinical Chemistry, Vol 5, 284-296, Copyright © 1959 by the American Association for Clinical Chemistry

Determination of Chloride in Blood Serum, Plasma, or Other Biologic Fluids by a New Rapid Precision Method

H. V. Malmstadt 1 and J. D. Winefordner 1

1 Department of Chemistry and Chemical Engineering, University of Illinois, Urbana, Ill.

The new, extremely sensitive and accurate precision null-point potentiometric method has been applied to the determination of chloride in blood serum or plasma and 2 simple procedures are described, one for deproteinized serum and the other for serum directly.

Measurements of chloride in diluted serum samples containing only 0.02 ml. blood serum can be carried out in less than 1 minute by the precision null-point potentiometric method with relative errors less than 0.5% for the complete procedure. Sample manipulations and all other operations for the chloride determination require about 1 to 3 minutes, the longer time for deproteinizing the serum. The same procedure is suitable for chloride determinations in other biologic samples, and the general considerations are presented.

Submitted on December 21, 1958






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Copyright © 1959 by the American Association for Clinical Chemistry.